Abstract
Many studies have analyzed myelin-reactivity of T cells in multiple sclerosis (MS); however, with conflicting results. In this study we compare methods to determine myelin reactivity of T cells and aim to delineate the cause of inconsistency in the literature. Challenging T cells with myelin antigens we found a significant increase in antigen-reactivity of T cells from patients with MS using an ELISpot-assay, in contrast to a CFSE-dilution assay. Comparing the two assays showed that the myelin-reactive T cells detected in the ELISpot-assay originated primarily from effector memory T cells in contrast to the myelin-reactive T cells of the CFSE-assay representing a population of both naive, central memory and effector memory T cells. This diversity in T cell populations activated in the two assays likely contribute to the discrepancy found in the literature and encourages thorough considerations when choosing an assay to determine antigen-specificity of T cells in future studies.
Original language | English |
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Article number | 108817 |
Journal | Clinical Immunology |
Volume | 230 |
Number of pages | 6 |
ISSN | 1521-6616 |
DOIs | |
Publication status | Published - 2021 |
Keywords
- Myelin-reactive T cells
- Assessing current methods
- Multiple sclerosis
- BASIC-PROTEIN
- OLIGODENDROCYTE GLYCOPROTEIN
- PROTEOLIPID PROTEIN
- CEREBROSPINAL-FLUID
- CYTOKINE PROFILE
- TARGET ANTIGENS
- FREQUENCY
- RESPONSES
- PEPTIDES
- BLOOD