Assessment of commonly used methods to determine myelin-reactivity of T cells in multiple sclerosis

Marina Rode von Essen*, Cecilie Ammitzboll, Lars Bornsen, Finn Sellebjerg

*Corresponding author for this work

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Abstract

Many studies have analyzed myelin-reactivity of T cells in multiple sclerosis (MS); however, with conflicting results. In this study we compare methods to determine myelin reactivity of T cells and aim to delineate the cause of inconsistency in the literature. Challenging T cells with myelin antigens we found a significant increase in antigen-reactivity of T cells from patients with MS using an ELISpot-assay, in contrast to a CFSE-dilution assay. Comparing the two assays showed that the myelin-reactive T cells detected in the ELISpot-assay originated primarily from effector memory T cells in contrast to the myelin-reactive T cells of the CFSE-assay representing a population of both naive, central memory and effector memory T cells. This diversity in T cell populations activated in the two assays likely contribute to the discrepancy found in the literature and encourages thorough considerations when choosing an assay to determine antigen-specificity of T cells in future studies.

Original languageEnglish
Article number108817
JournalClinical Immunology
Volume230
Number of pages6
ISSN1521-6616
DOIs
Publication statusPublished - 2021

Keywords

  • Myelin-reactive T cells
  • Assessing current methods
  • Multiple sclerosis
  • BASIC-PROTEIN
  • OLIGODENDROCYTE GLYCOPROTEIN
  • PROTEOLIPID PROTEIN
  • CEREBROSPINAL-FLUID
  • CYTOKINE PROFILE
  • TARGET ANTIGENS
  • FREQUENCY
  • RESPONSES
  • PEPTIDES
  • BLOOD

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