Azone® decreases the buccal mucosal permeation of Diazepam in a concentration-dependent manner via a reservoir effect

Emil Meng-Lund, Jette Jacobsen, Liang Jin, Christian Janfelt, René Holm, Anette Müllertz, Joseph A Nicolazzo

Research output: Contribution to journalJournal articleResearchpeer-review

13 Citations (Scopus)

Abstract

The purpose of this study was to examine concentration-dependent effects of Azone® (AZ) on the buccal absorption of diazepam (DIAZ). Porcine buccal mucosa was placed in modified Ussing chambers and pretreated with 10 μL of 0%, 5%, 20%, and 50% (w/v) AZ in ethanol. DIAZ was administered to the donor chamber either in solution or a chitosan-based gel. The donor chamber disappearance, receptor chamber appearance, and tissue retention of DIAZ were monitored over 2 h by HPLC, with AZ tissue disposition also measured by liquid chromatography-mass spectrometry profiling of tissue cryosections. DIAZ steady-state flux values significantly (p
Original languageEnglish
Article number103
JournalJournal of Pharmaceutical Sciences
Volume103
Issue number4
Pages (from-to)1133-1141
ISSN0022-3549
DOIs
Publication statusPublished - 15 Feb 2014

Cite this