Balanced callose and cellulose biosynthesis in Arabidopsis quorum sensing and pattern-triggered immunity

Xiaolin Liu, Zhiming Ma, Tuan Minh Tran, Carsten Rautengarten, Yingying Cheng, Liang Yang, Berit Ebert, Staffan Persson, Yansong Miao

Research output: Contribution to journalJournal articleResearchpeer-review

2 Citations (Scopus)
15 Downloads (Pure)

Abstract

The plant cell wall (CW) is one of the most important physical barriers phytopathogens must conquer to invade their hosts. This barrier is a dynamic structure that responds to pathogen infection through a complex network of immune receptors, together with CW-synthesizing and CW-degrading enzymes. Callose deposition in the primary CW is a well-known physical response to pathogen infection. Notably, callose and cellulose biosynthesis share an initial substrate, UDP-glucose, which is the main load-bearing component of the CW. However, how these two critical biosynthetic processes are balanced during plant-pathogen interactions remains unclear. Here, using two different pathogen-derived molecules, bacterial flagellin (flg22) and the diffusible signal factor (DSF) produced by Xanthomonas campestris pv. campestris, we show a negative correlation between cellulose and callose biosynthesis in Arabidopsis (Arabidopsis thaliana). By quantifying the abundance of callose and cellulose under DSF or flg22 elicitation and characterizing the dynamics of the enzymes involved in the biosynthesis and degradation of these two polymers, we show that the balance of these two CW components is mediated by the activity of a β-1,3-glucanase (BG2). Our data demonstrate balanced cellulose and callose biosynthesis during plant immune responses.

Original languageEnglish
JournalPlant Physiology
Volume194
Issue number1
Pages (from-to)137-152
ISSN0032-0889
DOIs
Publication statusPublished - 2024

Bibliographical note

© The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists.

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