Cancer-induced Bone Pain Impairs Burrowing Behaviour in Mouse and Rat

Sonny Hermanus Johannes Sliepen; Marta Diaz-Delcastillo; Johanna Korioth; Rikke Brix Olsen; Camilla Kristine Appel; Thomas Christoph; Anne-Marie Heegaard; Kris Rutten

doi:10.21873/invivo.11582

Cancer-induced Bone Pain Impairs Burrowing Behaviour in Mouse and Rat

Sonny Hermanus Johannes Sliepen, Marta Diaz-Delcastillo, Johanna Korioth, Rikke Brix Olsen, Camilla Kristine Appel, Thomas Christoph, Anne-Marie Heegaard, Kris Rutten

Translational and Clinical Pharmacology

Research output: Contribution to journal › Journal article › Research › peer-review

15 Citations (Scopus)

57 Downloads (Pure)

Abstract

BACKGROUND: Cancer-induced bone pain remains a serious public health concern, with a need for translational behavioural tests in order to assess nociception in preclinical models of this condition. Burrowing is an innate, ethologically relevant rodent behaviour that has been proven sensitive to chronic pain conditions. Herein, we studied for the first time whether burrowing performance is altered in preclinical models of cancer-induced bone pain.

MATERIALS AND METHODS: Mice and rats were inoculated with syngeneic breast cancer cells. Bone degradation was radiographically evaluated and nociception was assessed in limb-use and burrowing tests.

RESULTS: Cancer-bearing rodents showed reduced relative bone density and limb-use scores, confirming disease development. Burrowing performance decreased over time in both rodent models.

CONCLUSION: Burrowing performance was reduced in both rodent models, indicating that the burrowing test is a relevant and reproducible behavioural test for assessing disease development in both mouse and rat models of cancer-induced bone pain.

Original language	English
Journal	In Vivo
Volume	33
Issue number	4
Pages (from-to)	1125-1132
Number of pages	8
ISSN	0258-851X
DOIs	https://doi.org/10.21873/invivo.11582
Publication status	Published - 2019

Keywords

Animals
Behavior, Animal
Bone Neoplasms/complications
Cancer Pain/diagnosis
Case-Control Studies
Disease Models, Animal
Male
Mice
Pain Measurement/methods
Rats

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10.21873/invivo.11582Licence: CC BY

1125.fullFinal published version, 327 KBLicence: CC BY

Cite this

Cancer-induced Bone Pain Impairs Burrowing Behaviour in Mouse and Rat. / Sliepen, Sonny Hermanus Johannes; Diaz-Delcastillo, Marta; Korioth, Johanna; Olsen, Rikke Brix; Appel, Camilla Kristine; Christoph, Thomas; Heegaard, Anne-Marie; Rutten, Kris.

In: In Vivo, Vol. 33, No. 4, 2019, p. 1125-1132.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "Cancer-induced Bone Pain Impairs Burrowing Behaviour in Mouse and Rat",

abstract = "BACKGROUND: Cancer-induced bone pain remains a serious public health concern, with a need for translational behavioural tests in order to assess nociception in preclinical models of this condition. Burrowing is an innate, ethologically relevant rodent behaviour that has been proven sensitive to chronic pain conditions. Herein, we studied for the first time whether burrowing performance is altered in preclinical models of cancer-induced bone pain.MATERIALS AND METHODS: Mice and rats were inoculated with syngeneic breast cancer cells. Bone degradation was radiographically evaluated and nociception was assessed in limb-use and burrowing tests.RESULTS: Cancer-bearing rodents showed reduced relative bone density and limb-use scores, confirming disease development. Burrowing performance decreased over time in both rodent models.CONCLUSION: Burrowing performance was reduced in both rodent models, indicating that the burrowing test is a relevant and reproducible behavioural test for assessing disease development in both mouse and rat models of cancer-induced bone pain.",

keywords = "Animals, Behavior, Animal, Bone Neoplasms/complications, Cancer Pain/diagnosis, Case-Control Studies, Disease Models, Animal, Male, Mice, Pain Measurement/methods, Rats",

author = "Sliepen, {Sonny Hermanus Johannes} and Marta Diaz-Delcastillo and Johanna Korioth and Olsen, {Rikke Brix} and Appel, {Camilla Kristine} and Thomas Christoph and Anne-Marie Heegaard and Kris Rutten",

year = "2019",

doi = "10.21873/invivo.11582",

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AU - Sliepen, Sonny Hermanus Johannes

AU - Diaz-Delcastillo, Marta

AU - Korioth, Johanna

AU - Olsen, Rikke Brix

AU - Appel, Camilla Kristine

AU - Christoph, Thomas

AU - Heegaard, Anne-Marie

AU - Rutten, Kris

PY - 2019

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N2 - BACKGROUND: Cancer-induced bone pain remains a serious public health concern, with a need for translational behavioural tests in order to assess nociception in preclinical models of this condition. Burrowing is an innate, ethologically relevant rodent behaviour that has been proven sensitive to chronic pain conditions. Herein, we studied for the first time whether burrowing performance is altered in preclinical models of cancer-induced bone pain.MATERIALS AND METHODS: Mice and rats were inoculated with syngeneic breast cancer cells. Bone degradation was radiographically evaluated and nociception was assessed in limb-use and burrowing tests.RESULTS: Cancer-bearing rodents showed reduced relative bone density and limb-use scores, confirming disease development. Burrowing performance decreased over time in both rodent models.CONCLUSION: Burrowing performance was reduced in both rodent models, indicating that the burrowing test is a relevant and reproducible behavioural test for assessing disease development in both mouse and rat models of cancer-induced bone pain.

AB - BACKGROUND: Cancer-induced bone pain remains a serious public health concern, with a need for translational behavioural tests in order to assess nociception in preclinical models of this condition. Burrowing is an innate, ethologically relevant rodent behaviour that has been proven sensitive to chronic pain conditions. Herein, we studied for the first time whether burrowing performance is altered in preclinical models of cancer-induced bone pain.MATERIALS AND METHODS: Mice and rats were inoculated with syngeneic breast cancer cells. Bone degradation was radiographically evaluated and nociception was assessed in limb-use and burrowing tests.RESULTS: Cancer-bearing rodents showed reduced relative bone density and limb-use scores, confirming disease development. Burrowing performance decreased over time in both rodent models.CONCLUSION: Burrowing performance was reduced in both rodent models, indicating that the burrowing test is a relevant and reproducible behavioural test for assessing disease development in both mouse and rat models of cancer-induced bone pain.

KW - Animals

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KW - Cancer Pain/diagnosis

KW - Case-Control Studies

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KW - Mice

KW - Pain Measurement/methods

KW - Rats

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