Abstract
There is a growing interest in the use of cell-penetrating peptides (CPPs) as carriers for transepithelial drug delivery. This chapter gives an introduction to and discussion of the commonly used production and characterization methods for CPP–cargo samples including high-throughput cell viability screening. Moreover, we describe methods for permeation and cell viability assessment in the Caco-2 cell culture model with and without implementation of biosimilar mucus. Last, a method to assess metabolic degradation in vitro is described.
Translated title of the contribution | Celle penetrerende peptider som transepitheliel transportsystem for lægemidler in vitro |
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Original language | English |
Title of host publication | Cell-penetrating peptides : Methods and Protocols |
Editors | Ülo Langel |
Number of pages | 17 |
Volume | 1324 |
Publisher | Humana Press |
Publication date | 2015 |
Pages | 261-277 |
Chapter | 17 |
ISBN (Print) | 978-1-4939-2805-7 |
DOIs | |
Publication status | Published - 2015 |
Keywords
- Faculty of Health and Medical Sciences