Cell-penetrating peptides as carriers for transepithelial drug delivery in vitro

Stine Rønholt; Mie Kristensen; Hanne Mørck Nielsen

doi:10.1007/978-1-4939-2806-4

Cell-penetrating peptides as carriers for transepithelial drug delivery in vitro

Stine Rønholt, Mie Kristensen, Hanne Mørck Nielsen

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Abstract

There is a growing interest in the use of cell-penetrating peptides (CPPs) as carriers for transepithelial drug delivery. This chapter gives an introduction to and discussion of the commonly used production and characterization methods for CPP–cargo samples including high-throughput cell viability screening. Moreover, we describe methods for permeation and cell viability assessment in the Caco-2 cell culture model with and without implementation of biosimilar mucus. Last, a method to assess metabolic degradation in vitro is described.

Translated title of the contribution	Celle penetrerende peptider som transepitheliel transportsystem for lægemidler in vitro
Original language	English
Title of host publication	Cell-penetrating peptides : Methods and Protocols
Editors	Ülo Langel
Number of pages	17
Volume	1324
Publisher	Humana Press
Publication date	2015
Pages	261-277
Chapter	17
ISBN (Print)	978-1-4939-2805-7
DOIs	https://doi.org/10.1007/978-1-4939-2806-4
Publication status	Published - 2015

Keywords

Faculty of Health and Medical Sciences

Access to Document

10.1007/978-1-4939-2806-4

Cite this

@inbook{0eebb54bf6c44950aff7fff1f1e95cf6,

title = "Cell-penetrating peptides as carriers for transepithelial drug delivery in vitro",

abstract = "There is a growing interest in the use of cell-penetrating peptides (CPPs) as carriers for transepithelial drug delivery. This chapter gives an introduction to and discussion of the commonly used production and characterization methods for CPP–cargo samples including high-throughput cell viability screening. Moreover, we describe methods for permeation and cell viability assessment in the Caco-2 cell culture model with and without implementation of biosimilar mucus. Last, a method to assess metabolic degradation in vitro is described.",

keywords = "Faculty of Health and Medical Sciences, Biologics, Peptides, Drug delivery, Transepithel, in vitro, Cell-penetrating peptides, CPP, proteins, Methods, Protocols, Complexation, Microfluidics, Biophysics, Caco-2 cell model, Caco-2, Formulation, Drug formulation",

author = "Stine R{\o}nholt and Mie Kristensen and Nielsen, {Hanne M{\o}rck}",

year = "2015",

doi = "10.1007/978-1-4939-2806-4",

language = "English",

isbn = "978-1-4939-2805-7",

volume = "1324",

pages = "261--277",

editor = "Langel, {{\"U}lo }",

booktitle = "Cell-penetrating peptides",

publisher = "Humana Press",

address = "United States",

}

TY - CHAP

T1 - Cell-penetrating peptides as carriers for transepithelial drug delivery in vitro

AU - Rønholt, Stine

AU - Kristensen, Mie

AU - Nielsen, Hanne Mørck

PY - 2015

Y1 - 2015

N2 - There is a growing interest in the use of cell-penetrating peptides (CPPs) as carriers for transepithelial drug delivery. This chapter gives an introduction to and discussion of the commonly used production and characterization methods for CPP–cargo samples including high-throughput cell viability screening. Moreover, we describe methods for permeation and cell viability assessment in the Caco-2 cell culture model with and without implementation of biosimilar mucus. Last, a method to assess metabolic degradation in vitro is described.

AB - There is a growing interest in the use of cell-penetrating peptides (CPPs) as carriers for transepithelial drug delivery. This chapter gives an introduction to and discussion of the commonly used production and characterization methods for CPP–cargo samples including high-throughput cell viability screening. Moreover, we describe methods for permeation and cell viability assessment in the Caco-2 cell culture model with and without implementation of biosimilar mucus. Last, a method to assess metabolic degradation in vitro is described.

KW - Faculty of Health and Medical Sciences

KW - Biologics

KW - Peptides

KW - Drug delivery

KW - Transepithel

KW - in vitro

KW - Cell-penetrating peptides

KW - CPP

KW - proteins

KW - Methods

KW - Protocols

KW - Complexation

KW - Microfluidics

KW - Biophysics

KW - Caco-2 cell model

KW - Caco-2

KW - Formulation

KW - Drug formulation

UR - http://link.springer.com/protocol/10.1007/978-1-4939-2806-4_17

U2 - 10.1007/978-1-4939-2806-4

DO - 10.1007/978-1-4939-2806-4

M3 - Book chapter

SN - 978-1-4939-2805-7

VL - 1324

SP - 261

EP - 277

BT - Cell-penetrating peptides

A2 - Langel, Ülo

PB - Humana Press

ER -