Cell-penetrating peptides as carriers for transepithelial drug delivery in vitro

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Abstract

There is a growing interest in the use of cell-penetrating peptides (CPPs) as carriers for transepithelial drug delivery. This chapter gives an introduction to and discussion of the commonly used production and characterization methods for CPP–cargo samples including high-throughput cell viability screening. Moreover, we describe methods for permeation and cell viability assessment in the Caco-2 cell culture model with and without implementation of biosimilar mucus. Last, a method to assess metabolic degradation in vitro is described.
Translated title of the contributionCelle penetrerende peptider som transepitheliel transportsystem for lægemidler in vitro
Original languageEnglish
Title of host publicationCell-penetrating peptides : Methods and Protocols
EditorsÜlo Langel
Number of pages17
Volume1324
PublisherHumana Press
Publication date2015
Pages261-277
Chapter17
ISBN (Print)978-1-4939-2805-7
DOIs
Publication statusPublished - 2015

Keywords

  • Faculty of Health and Medical Sciences

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