Characterization of the gut bacterial and viral microbiota in latent autoimmune diabetes in adults

Casper S. Poulsen, Dan Hesse, Gabriel R. Fernandes, Tue H. Hansen, Timo Kern, Allan Linneberg, Lore Van Espen, Torben Jørgensen, Trine Nielsen, Amra C. Alibegovic, Jelle Matthijnssens, Oluf Pedersen, Henrik Vestergaard, Torben Hansen*, Mette K. Andersen

*Corresponding author for this work

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Abstract

Latent autoimmune diabetes in adults (LADA) is a heterogeneous disease characterized by autoantibodies against insulin producing pancreatic beta cells and initial lack of need for insulin treatment. The aim of the present study was to investigate if individuals with LADA have an altered gut microbiota relative to non-diabetic control subjects, individuals with type 1 diabetes (T1D), and individuals with type 2 diabetes (T2D). Bacterial community profiling was performed with primers targeting the variable region 4 of the 16S rRNA gene and sequenced. Amplicon sequence variants (ASVs) were generated with DADA2 and annotated to the SILVA database. The gut virome was sequenced, using a viral particle enrichment and metagenomics approach, assembled, and quantified to describe the composition of the viral community. Comparison of the bacterial alpha- and beta-diversity measures revealed that the gut bacteriome of individuals with LADA resembled that of individuals with T2D. Yet, specific genera were found to differ in abundance in individuals with LADA compared with T1D and T2D, indicating that LADA has unique taxonomical features. The virome composition reflected the stability of the most dominant order Caudovirales and the families Siphoviridae, Podoviridae, and Inoviridae, and the dominant family Microviridae. Further studies are needed to confirm these findings.

Original languageEnglish
Article number8315
JournalScientific Reports
Volume14
Issue number1
Number of pages11
ISSN2045-2322
DOIs
Publication statusPublished - 2024

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