Colorimetric pH indicators based on well-defined amylose and amylopectin matrices enriched with anthocyanins from red cabbage

Marwa Faisal, Marta Bevilacqua*, Rasmus Bro, Heloisa N. Bordallo, Jacob Judas Kain Kirkensgaard, Kim H. Hebelstrup, Andreas Blennow*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

8 Citations (Scopus)

Abstract

This study aimed to prepare a novel colorimetric indicator film from virtually pure (99 %) amylose (AM) and anthocyanins extracted from red cabbage (RCA). The AM used was a unique engineered bulk material extracted from transgenic barley grains. Films produced by solution casting were compared to normal barely starch (NB) and pure barley amylopectin (AP), with amylose contents of 30 % and 0 %, respectively. The pH-indicator films were produced by incorporation of RCA into the different starch support matrices with different amylose contents. Barrier, thermal, and mechanical properties, photo degradation stability, and release behavior data revealed that RCA interact differently through the glucan matrices. Microstructural observations showed that RCA were evenly dispersed in the glucan matrix, and AM+RCA indicator films showed high UV-barrier and mechanical performance over normal starch. FTIR revealed that RCA was properly affected by the AM matrix. Moreover, the AM+RCA films showed sensitive color changes in the pH range (2–11) and a predominant Fickian diffusion release mechanism for RCA. This study provides for the first time data regarding AM films with RCA and their promising potential for application as support matrices in responsive food and other industrial biodegradable packaging materials.

Original languageEnglish
Article number126250
JournalInternational Journal of Biological Macromolecules
Volume250
Number of pages13
ISSN0141-8130
DOIs
Publication statusPublished - 2023

Bibliographical note

Publisher Copyright:
© 2023

Keywords

  • Amylopectin
  • Amylose
  • Anthocyanins
  • pH-indicator
  • Responsive packaging

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