Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain

Jenny M. Brown; Bao Anh Phan; Nadia Aalling; Gregory J. Morton; Michael W. Schwartz; Jarrad M. Scarlett

doi:10.1016/j.xpro.2022.101329

Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain

Jenny M. Brown^*, Bao Anh Phan, Nadia Aalling, Gregory J. Morton, Michael W. Schwartz, Jarrad M. Scarlett

^*Corresponding author for this work

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Abstract

Intracerebroventricular (icv) injection of fibroblast growth factor 1 (FGF1) elicits remission of diabetic hyperglycemia in rodent models of type 2 diabetes. Here, we present an optimized protocol to study the intracellular signaling pathways underlying the FGF1-induced sustained glucose lowering in the mouse brain. This protocol combines icv injection of FGF1 and osmotic mini-pump infusion of U0126, an inhibitor of MAPK/ERK signaling. We describe the surgical procedure and verification of U0126 inhibition of FGF1-stimulated hypothalamic MAPK/ERK signaling via western blot. For complete details on the use and execution of this protocol, please refer to Brown et al. (2021).

Original language	English
Article number	101329
Journal	STAR Protocols
Volume	3
Issue number	2
Number of pages	16
ISSN	2666-1667
DOIs	https://doi.org/10.1016/j.xpro.2022.101329
Publication status	Published - 2022

Bibliographical note

Publisher Copyright:
© 2022 The Author(s)

Keywords

Metabolism
Model Organisms
Molecular Biology
Neuroscience
Signal Transduction

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Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain. / Brown, Jenny M.; Phan, Bao Anh; Aalling, Nadia; Morton, Gregory J.; Schwartz, Michael W.; Scarlett, Jarrad M.

In: STAR Protocols, Vol. 3, No. 2, 101329, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

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abstract = "Intracerebroventricular (icv) injection of fibroblast growth factor 1 (FGF1) elicits remission of diabetic hyperglycemia in rodent models of type 2 diabetes. Here, we present an optimized protocol to study the intracellular signaling pathways underlying the FGF1-induced sustained glucose lowering in the mouse brain. This protocol combines icv injection of FGF1 and osmotic mini-pump infusion of U0126, an inhibitor of MAPK/ERK signaling. We describe the surgical procedure and verification of U0126 inhibition of FGF1-stimulated hypothalamic MAPK/ERK signaling via western blot. For complete details on the use and execution of this protocol, please refer to Brown et al. (2021).",

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AU - Morton, Gregory J.

AU - Schwartz, Michael W.

AU - Scarlett, Jarrad M.

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N2 - Intracerebroventricular (icv) injection of fibroblast growth factor 1 (FGF1) elicits remission of diabetic hyperglycemia in rodent models of type 2 diabetes. Here, we present an optimized protocol to study the intracellular signaling pathways underlying the FGF1-induced sustained glucose lowering in the mouse brain. This protocol combines icv injection of FGF1 and osmotic mini-pump infusion of U0126, an inhibitor of MAPK/ERK signaling. We describe the surgical procedure and verification of U0126 inhibition of FGF1-stimulated hypothalamic MAPK/ERK signaling via western blot. For complete details on the use and execution of this protocol, please refer to Brown et al. (2021).

AB - Intracerebroventricular (icv) injection of fibroblast growth factor 1 (FGF1) elicits remission of diabetic hyperglycemia in rodent models of type 2 diabetes. Here, we present an optimized protocol to study the intracellular signaling pathways underlying the FGF1-induced sustained glucose lowering in the mouse brain. This protocol combines icv injection of FGF1 and osmotic mini-pump infusion of U0126, an inhibitor of MAPK/ERK signaling. We describe the surgical procedure and verification of U0126 inhibition of FGF1-stimulated hypothalamic MAPK/ERK signaling via western blot. For complete details on the use and execution of this protocol, please refer to Brown et al. (2021).

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