Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides

E Nordhoff; F Kirpekar; M Karas; R Cramer; S Hahner; F Hillenkamp; K Kristiansen; P Roepstroff; A Lezius

Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides

E Nordhoff, F Kirpekar, M Karas, R Cramer, S Hahner, F Hillenkamp, K Kristiansen, P Roepstroff, A Lezius

Research output: Contribution to journal › Journal article › Research › peer-review

69 Citations (Scopus)

Abstract

UV-matrix assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) with 3-hydroxypicolinic acid as matrix and IR-MALDI-MS with succinic acid as matrix have proved their feasibility for highly accurate and sensitive mass determination of nucleic acids (DNA and RNA). In this work, a detailed comparison of these two MALDI-methods and between positive- and negative ion mass spectra for the analysis of oligodeoxynucleotides is undertaken. Mass spectra of DNA sequences with up to 40 nucleotides are shown. Both linear and reflectron time-of-flight mass analyzers were used within this study and are compared for their potential in the MALDI analysis of oligodeoxynucleotides. The role of molecule-ion fragmentation is also discussed.

Original language	English
Journal	Nucleic Acids Research
Volume	22
Issue number	13
Pages (from-to)	2460-5
Number of pages	5
ISSN	0305-1048
Publication status	Published - 1994
Externally published	Yes

Bibliographical note

Keywords: Base Sequence; Lasers; Mass Spectrometry; Molecular Sequence Data; Oligodeoxyribonucleotides; Picolinic Acids; Spectrophotometry, Infrared; Spectrophotometry, Ultraviolet; Succinates; Succinic Acid

Access to Document

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=308196

Cite this

Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides. / Nordhoff, E; Kirpekar, F; Karas, M; Cramer, R; Hahner, S; Hillenkamp, F; Kristiansen, K; Roepstroff, P; Lezius, A.

In: Nucleic Acids Research, Vol. 22, No. 13, 1994, p. 2460-5.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides",

abstract = "UV-matrix assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) with 3-hydroxypicolinic acid as matrix and IR-MALDI-MS with succinic acid as matrix have proved their feasibility for highly accurate and sensitive mass determination of nucleic acids (DNA and RNA). In this work, a detailed comparison of these two MALDI-methods and between positive- and negative ion mass spectra for the analysis of oligodeoxynucleotides is undertaken. Mass spectra of DNA sequences with up to 40 nucleotides are shown. Both linear and reflectron time-of-flight mass analyzers were used within this study and are compared for their potential in the MALDI analysis of oligodeoxynucleotides. The role of molecule-ion fragmentation is also discussed.",

author = "E Nordhoff and F Kirpekar and M Karas and R Cramer and S Hahner and F Hillenkamp and K Kristiansen and P Roepstroff and A Lezius",

note = "Keywords: Base Sequence; Lasers; Mass Spectrometry; Molecular Sequence Data; Oligodeoxyribonucleotides; Picolinic Acids; Spectrophotometry, Infrared; Spectrophotometry, Ultraviolet; Succinates; Succinic Acid",

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T1 - Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides

AU - Nordhoff, E

AU - Kirpekar, F

AU - Karas, M

AU - Cramer, R

AU - Hahner, S

AU - Hillenkamp, F

AU - Kristiansen, K

AU - Roepstroff, P

AU - Lezius, A

N1 - Keywords: Base Sequence; Lasers; Mass Spectrometry; Molecular Sequence Data; Oligodeoxyribonucleotides; Picolinic Acids; Spectrophotometry, Infrared; Spectrophotometry, Ultraviolet; Succinates; Succinic Acid

PY - 1994

Y1 - 1994

N2 - UV-matrix assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) with 3-hydroxypicolinic acid as matrix and IR-MALDI-MS with succinic acid as matrix have proved their feasibility for highly accurate and sensitive mass determination of nucleic acids (DNA and RNA). In this work, a detailed comparison of these two MALDI-methods and between positive- and negative ion mass spectra for the analysis of oligodeoxynucleotides is undertaken. Mass spectra of DNA sequences with up to 40 nucleotides are shown. Both linear and reflectron time-of-flight mass analyzers were used within this study and are compared for their potential in the MALDI analysis of oligodeoxynucleotides. The role of molecule-ion fragmentation is also discussed.

AB - UV-matrix assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) with 3-hydroxypicolinic acid as matrix and IR-MALDI-MS with succinic acid as matrix have proved their feasibility for highly accurate and sensitive mass determination of nucleic acids (DNA and RNA). In this work, a detailed comparison of these two MALDI-methods and between positive- and negative ion mass spectra for the analysis of oligodeoxynucleotides is undertaken. Mass spectra of DNA sequences with up to 40 nucleotides are shown. Both linear and reflectron time-of-flight mass analyzers were used within this study and are compared for their potential in the MALDI analysis of oligodeoxynucleotides. The role of molecule-ion fragmentation is also discussed.

M3 - Journal article

C2 - 8041606

VL - 22

SP - 2460

EP - 2465

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 13

ER -