Comparison of methods and animal models commonly used for investigation of fecal microbiota: effects of time, host and gender

Nete Bernbom; Birgit Nørrung; Peter Saadbye; Lars Mølbak; Finn Kvist Vogensen; Tine Rask Licht

doi:10.1016/j.mimet.2005.10.014

Comparison of methods and animal models commonly used for investigation of fecal microbiota: effects of time, host and gender

Nete Bernbom, Birgit Nørrung, Peter Saadbye, Lars Mølbak, Finn Kvist Vogensen, Tine Rask Licht

Research output: Contribution to journal › Journal article › Research › peer-review

37 Citations (Scopus)

Abstract

Denaturing gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (T-RFLP) and plating on selective agars were used to study variation in the fecal microbiota of rats over time as well as variation between individuals. Investigated rats were either conventional and specific pathogen free (SPF), or human flora associated (HFA). A higher variation (p < 0.05) in fecal microbiota over time was observed for HFA than for SPF animals. Analysis of DGGE and T-RFLP profiles of fecal microbiota from SPF and HFA rats revealed that variation over time was less significant than variation between individuals, and that phylogenetic profiles clustered according to gender. These observations should be taken into account when designing future research addressing changes in fecal microbiota.

Original language	English
Journal	Journal of Microbiological Methods
Volume	66
Issue number	1
Pages (from-to)	87-95
Number of pages	9
ISSN	0167-7012
DOIs	https://doi.org/10.1016/j.mimet.2005.10.014
Publication status	Published - 2006

Keywords

Former LIFE faculty
DGGE; T-RFLP; Fecal microbiota; Community analysis

Access to Document

10.1016/j.mimet.2005.10.014

Cite this

Comparison of methods and animal models commonly used for investigation of fecal microbiota : effects of time, host and gender. / Bernbom, Nete; Nørrung, Birgit; Saadbye, Peter; Mølbak, Lars; Vogensen, Finn Kvist; Licht, Tine Rask.

In: Journal of Microbiological Methods, Vol. 66, No. 1, 2006, p. 87-95.

Research output: Contribution to journal › Journal article › Research › peer-review

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AU - Mølbak, Lars

AU - Vogensen, Finn Kvist

AU - Licht, Tine Rask

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