TY - JOUR
T1 - Complement split product C3c in saliva as biomarker for periodontitis and response to periodontal treatment
AU - Grande, Maria Anastasia
AU - Belstrøm, Daniel
AU - Damgaard, Christian
AU - Holmstrup, Palle
AU - Thangaraj, Sai Sindhu
AU - Nielsen, Claus Henrik
AU - Palarasah, Yaseelan
PY - 2021
Y1 - 2021
N2 - Background and Objective The complement system is engaged in inflammatory reactions both in the periodontal pockets and in the periodontium itself, where it can mediate tissue destruction. The aim of this study was, first, to compare salivary levels of the total complement system protein C3 and its split product, fluid-phase C3c in patients with periodontitis and periodontally healthy controls. Next, to determine if C3 and C3c levels had biomarker potential in diagnosing and monitoring periodontitis and its treatment. We hypothesized that salivary levels of total C3 and the split product C3c associated with the severity of periodontitis and reflected decreased inflammatory activity after periodontal treatment. Methods At baseline, stimulated saliva samples were collected from patients with periodontitis (n = 18) and periodontally healthy controls (n = 15). Subsequently, non-surgical periodontal treatment was performed in the patients, and saliva sampling from patients was repeated two-, six-, and twelve weeks post-treatment (NCT02913248 at clinicaltrials.gov). The patients were grouped as good and poor responders to treatment according to the achieved reduction in bleeding on probing (BOP). Salivary levels of C3 and C3c were quantified using sandwich ELISA. Results Patients with periodontitis had higher baseline levels of both total C3 and the split product C3c in saliva than did periodontally healthy controls (P <.0001). Receiver operating curve (ROC) analyses discriminated patients with periodontitis from controls based on both C3 (AUC (area under curve) = 0.91,P <.001) and C3c levels (AUC = 0.84,P <.001) in saliva. Periodontal treatment improved all clinical parameters (P <.01). Good responders (n = 10) had lower baseline levels of C3c than poor responders (n = 8), (P <.05), and baseline levels of C3c discriminated between good and poor responders (AUC = 0.80,P <.05). Conclusion In conclusion, patients with periodontitis had higher salivary levels of C3c, and the C3c levels were predictive of reductions in BOP, that is, the poor responders. This suggests that salivary C3c levels possess potential to serve as a biomarker predicting the clinical response to non-surgical periodontal treatment.
AB - Background and Objective The complement system is engaged in inflammatory reactions both in the periodontal pockets and in the periodontium itself, where it can mediate tissue destruction. The aim of this study was, first, to compare salivary levels of the total complement system protein C3 and its split product, fluid-phase C3c in patients with periodontitis and periodontally healthy controls. Next, to determine if C3 and C3c levels had biomarker potential in diagnosing and monitoring periodontitis and its treatment. We hypothesized that salivary levels of total C3 and the split product C3c associated with the severity of periodontitis and reflected decreased inflammatory activity after periodontal treatment. Methods At baseline, stimulated saliva samples were collected from patients with periodontitis (n = 18) and periodontally healthy controls (n = 15). Subsequently, non-surgical periodontal treatment was performed in the patients, and saliva sampling from patients was repeated two-, six-, and twelve weeks post-treatment (NCT02913248 at clinicaltrials.gov). The patients were grouped as good and poor responders to treatment according to the achieved reduction in bleeding on probing (BOP). Salivary levels of C3 and C3c were quantified using sandwich ELISA. Results Patients with periodontitis had higher baseline levels of both total C3 and the split product C3c in saliva than did periodontally healthy controls (P <.0001). Receiver operating curve (ROC) analyses discriminated patients with periodontitis from controls based on both C3 (AUC (area under curve) = 0.91,P <.001) and C3c levels (AUC = 0.84,P <.001) in saliva. Periodontal treatment improved all clinical parameters (P <.01). Good responders (n = 10) had lower baseline levels of C3c than poor responders (n = 8), (P <.05), and baseline levels of C3c discriminated between good and poor responders (AUC = 0.80,P <.05). Conclusion In conclusion, patients with periodontitis had higher salivary levels of C3c, and the C3c levels were predictive of reductions in BOP, that is, the poor responders. This suggests that salivary C3c levels possess potential to serve as a biomarker predicting the clinical response to non-surgical periodontal treatment.
KW - complement component 3
KW - intervention
KW - periodontitis
KW - saliva
KW - GINGIVAL FLUID
KW - MONOCLONAL-ANTIBODY
KW - COMPONENTS
KW - CLEAVAGE
KW - DISEASES
KW - MARKERS
KW - SYSTEM
KW - SERUM
U2 - 10.1111/jre.12788
DO - 10.1111/jre.12788
M3 - Journal article
C2 - 32681659
VL - 56
SP - 27
EP - 33
JO - Journal of Periodontal Research
JF - Journal of Periodontal Research
SN - 0022-3484
IS - 1
ER -