TY - JOUR
T1 - Complete genome sequence of the cystic fibrosis pathogen Achromobacter xylosoxidans NH44784-1996 complies with important pathogenic phenotypes
AU - Jakobsen, Tim Holm
AU - Hansen, Martin Asser
AU - Jensen, Peter Østrup
AU - Hansen, Lars
AU - Riber, Leise
AU - Cockburn, April Patricia Indera
AU - Kolpen, Mette
AU - Hansen, Christine Rønne
AU - Ridderberg, Winnie
AU - Eickhardt-Sørensen, Steffen Robert
AU - Hansen, Marlene
AU - Kerpedjiev, Peter
AU - Alhede, Morten
AU - Qvortrup, Klaus
AU - Burmølle, Mette
AU - Moser, Claus Ernst
AU - Kühl, Michael
AU - Ciofu, Oana
AU - Givskov, Michael
AU - Sørensen, Søren Johannes
AU - Høiby, Niels
AU - Bjarnsholt, Thomas
PY - 2013
Y1 - 2013
N2 - Achromobacter xylosoxidans is an environmental opportunistic pathogen, which infects an increasing number of immunocompromised patients. In this study we combined genomic analysis of a clinical isolated A. xylosoxidans strain with phenotypic investigations of its important pathogenic features. We present a complete assembly of the genome of A. xylosoxidans NH44784-1996, an isolate from a cystic fibrosis patient obtained in 1996. The genome of A. xylosoxidans NH44784-1996 contains approximately 7 million base pairs with 6390 potential protein-coding sequences. We identified several features that render it an opportunistic human pathogen, We found genes involved in anaerobic growth and the pgaABCD operon encoding the biofilm adhesin poly-β-1,6-N-acetyl-D-glucosamin. Furthermore, the genome contains a range of antibiotic resistance genes coding efflux pump systems and antibiotic modifying enzymes. In vitro studies of A. xylosoxidans NH44784-1996 confirmed the genomic evidence for its ability to form biofilms, anaerobic growth via denitrification, and resistance to a broad range of antibiotics. Our investigation enables further studies of the functionality of important identified genes contributing to the pathogenicity of A. xylosoxidans and thereby improves our understanding and ability to treat this emerging pathogen.
AB - Achromobacter xylosoxidans is an environmental opportunistic pathogen, which infects an increasing number of immunocompromised patients. In this study we combined genomic analysis of a clinical isolated A. xylosoxidans strain with phenotypic investigations of its important pathogenic features. We present a complete assembly of the genome of A. xylosoxidans NH44784-1996, an isolate from a cystic fibrosis patient obtained in 1996. The genome of A. xylosoxidans NH44784-1996 contains approximately 7 million base pairs with 6390 potential protein-coding sequences. We identified several features that render it an opportunistic human pathogen, We found genes involved in anaerobic growth and the pgaABCD operon encoding the biofilm adhesin poly-β-1,6-N-acetyl-D-glucosamin. Furthermore, the genome contains a range of antibiotic resistance genes coding efflux pump systems and antibiotic modifying enzymes. In vitro studies of A. xylosoxidans NH44784-1996 confirmed the genomic evidence for its ability to form biofilms, anaerobic growth via denitrification, and resistance to a broad range of antibiotics. Our investigation enables further studies of the functionality of important identified genes contributing to the pathogenicity of A. xylosoxidans and thereby improves our understanding and ability to treat this emerging pathogen.
U2 - 10.1371/journal.pone.0068484
DO - 10.1371/journal.pone.0068484
M3 - Journal article
C2 - 23894309
VL - 8
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 7
M1 - e68484
ER -