Complete sequence of the extrachromosomal rDNA molecule from the ciliate Tetrahymena thermophila strain B1868VII

J Engberg; Henrik Nielsen

Complete sequence of the extrachromosomal rDNA molecule from the ciliate Tetrahymena thermophila strain B1868VII

J Engberg
, Henrik Nielsen

Department of Cellular and Molecular Medicine

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The recent development of rDNA vectors for transformation of Tetrahymena combined with improved microinjection technology should lead to a renewed interest in this organism. In particular, the rDNA itself constitutes an attractive system for biochemical studies. The rDNA is amplified to a level of 2% of the total DNA and exists as extrachromosomal molecules. Furthermore, the rDNA is homogeneous in sequence because it is derived from a single gene during sexual reorganization. In order to facilitate studies of this molecule, we report here a compilation of previously published sequence information together with new sequence data that completes the entire sequence of the 21 kb rDNA molecule.

Original language	English
Journal	Nucleic Acids Research
Volume	18
Issue number	23
Pages (from-to)	6915-9
Number of pages	4
ISSN	0305-1048
Publication status	Published - 1990

Bibliographical note

Keywords: Animals; Base Sequence; DNA, Ribosomal; Extrachromosomal Inheritance; Molecular Sequence Data; Sequence Homology, Nucleic Acid; Tetrahymena; Transcription, Genetic

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title = "Complete sequence of the extrachromosomal rDNA molecule from the ciliate Tetrahymena thermophila strain B1868VII",

abstract = "The recent development of rDNA vectors for transformation of Tetrahymena combined with improved microinjection technology should lead to a renewed interest in this organism. In particular, the rDNA itself constitutes an attractive system for biochemical studies. The rDNA is amplified to a level of 2\% of the total DNA and exists as extrachromosomal molecules. Furthermore, the rDNA is homogeneous in sequence because it is derived from a single gene during sexual reorganization. In order to facilitate studies of this molecule, we report here a compilation of previously published sequence information together with new sequence data that completes the entire sequence of the 21 kb rDNA molecule.",

author = "J Engberg and Henrik Nielsen",

note = "Keywords: Animals; Base Sequence; DNA, Ribosomal; Extrachromosomal Inheritance; Molecular Sequence Data; Sequence Homology, Nucleic Acid; Tetrahymena; Transcription, Genetic",

year = "1990",

language = "English",

volume = "18",

pages = "6915--9",

journal = "Nucleic Acids Research",

issn = "0305-1048",

publisher = "Oxford University Press",

number = "23",

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TY - JOUR

T1 - Complete sequence of the extrachromosomal rDNA molecule from the ciliate Tetrahymena thermophila strain B1868VII

AU - Engberg, J

AU - Nielsen, Henrik

N1 - Keywords: Animals; Base Sequence; DNA, Ribosomal; Extrachromosomal Inheritance; Molecular Sequence Data; Sequence Homology, Nucleic Acid; Tetrahymena; Transcription, Genetic

PY - 1990

Y1 - 1990

N2 - The recent development of rDNA vectors for transformation of Tetrahymena combined with improved microinjection technology should lead to a renewed interest in this organism. In particular, the rDNA itself constitutes an attractive system for biochemical studies. The rDNA is amplified to a level of 2% of the total DNA and exists as extrachromosomal molecules. Furthermore, the rDNA is homogeneous in sequence because it is derived from a single gene during sexual reorganization. In order to facilitate studies of this molecule, we report here a compilation of previously published sequence information together with new sequence data that completes the entire sequence of the 21 kb rDNA molecule.

AB - The recent development of rDNA vectors for transformation of Tetrahymena combined with improved microinjection technology should lead to a renewed interest in this organism. In particular, the rDNA itself constitutes an attractive system for biochemical studies. The rDNA is amplified to a level of 2% of the total DNA and exists as extrachromosomal molecules. Furthermore, the rDNA is homogeneous in sequence because it is derived from a single gene during sexual reorganization. In order to facilitate studies of this molecule, we report here a compilation of previously published sequence information together with new sequence data that completes the entire sequence of the 21 kb rDNA molecule.

M3 - Journal article

C2 - 2263454

SN - 0305-1048

VL - 18

SP - 6915

EP - 6919

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 23

ER -