CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis

Katharina Kohm; Veronika Theresa Lutz; Ines Friedrich; Robert Hertel

doi:10.1007/978-1-0716-2795-2_15

CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis

Katharina Kohm, Veronika Theresa Lutz, Ines Friedrich, Robert Hertel^*

^*Corresponding author for this work

Food Safety and Zoonoses

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Abstract

Phages are viruses of bacteria and have been known for over a century. They do not have a metabolism or protein synthesis machinery and rely on host cells for replication. The model organism Bacillus subtilis has served as a host strain for decades and enabled the isolation of many unique viral strains. However, many viral species representatives remained orphans as no, or only a few, related phages were ever re-isolated. The presented protocol describes how a CRISPR-Cas9 system with an artificial CRISPR-array can be set up and used to discriminate abundant and well-known B. subtilis phage from a host-based metagenome enrichment. The obtained viral suspension can be used for metagenome sequencing and isolating new viral strains.

Original language	English
Title of host publication	Metagenomics : Methods and Protocols
Editors	Wolfgang R. Streit, Rolf Daniel
Number of pages	8
Publisher	Humana Press
Publication date	2023
Pages	205-212
ISBN (Print)	978-1-0716-2797-6
ISBN (Electronic)	978-1-0716-2795-2
DOIs	https://doi.org/10.1007/978-1-0716-2795-2_15
Publication status	Published - 2023

Series	Methods in Molecular Biology
Volume	2555
ISSN	1064-3745

Bibliographical note

Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Bacillus subtilis
Bacteriophage
CRISPR-Cas9
CutSPR
gRNA
Metagenome
Phage

Access to Document

10.1007/978-1-0716-2795-2_15

Cite this

CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis. / Kohm, Katharina; Lutz, Veronika Theresa; Friedrich, Ines; Hertel, Robert.

Metagenomics: Methods and Protocols. ed. / Wolfgang R. Streit; Rolf Daniel. Humana Press, 2023. p. 205-212 (Methods in Molecular Biology, Vol. 2555).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

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