Cultivated single stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA binding stains

Karin Holmfeldt; Dusko Odic; Matthew B. Sullivan; Mathias Middelboe; Lasse Riemann

doi:10.1128/AEM.06580-11

Cultivated single stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA binding stains

Karin Holmfeldt, Dusko Odic, Matthew B. Sullivan, Mathias Middelboe, Lasse Riemann

Marine Biology

Research output: Contribution to journal › Journal article › Research › peer-review

46 Citations (Scopus)

Abstract

This is the first description of cultivated icosahedral single stranded DNA (ssDNA) phages isolated on heterotrophic marine bacterioplankton and with Bacteroidetes hosts. None of the 8 phages stained well with DNA binding stains, suggesting that in situ abundances of ssDNA phages are drastically underestimated using conventional methods for enumeration.

Original language	English
Journal	Applied and Environmental Microbiology
Volume	78
Issue number	3
Pages (from-to)	892-894
ISSN	0099-2240
DOIs	https://doi.org/10.1128/AEM.06580-11
Publication status	Published - 2012

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10.1128/AEM.06580-11

Cultivated single stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA binding stainsFinal published version, 559 KB

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Cultivated single stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA binding stains. / Holmfeldt, Karin; Odic, Dusko; Sullivan, Matthew B.; Middelboe, Mathias ; Riemann, Lasse.

In: Applied and Environmental Microbiology, Vol. 78, No. 3, 2012, p. 892-894.

Research output: Contribution to journal › Journal article › Research › peer-review

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