Danish apple cultivars: genetic diversity, parentage and breeding potential

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Abstract

Abstract
We made a genetic analysis of about 500 apple cultivars, which either originate
from Denmark or have been of greater importance in Denmark. To study the diversity and parentage among the cultivars, 15 SSR markers were used. A new PCR-based protocol for identification of S-alleles was developed and used to study the S-alleles among cultivars. The protocol using general S-locus primers and digestion with two restriction enzymes, for fragment analysis on an automated DNA sequencer fastens the process and gives an accurate estimation of fragment lengths. The ploidy level was determined using flow-cytometry. All cultivars were distinguishable by the SSRmarkers though Danish originating cultivars did not form a group distinct from foreign cultivars. The parentage of several well-known cultivars was revealed.
Original languageEnglish
Title of host publicationProceedings of the XIV EUCARPIA Symposium on Fruit Breeding and Genetics
Number of pages4
PublisherInternational Society for Horticultural Science
Publication date2017
Pages355-358
DOIs
Publication statusPublished - 2017
EventEucarpia Symposium on Fruit Breeding and Genetics 2015 - Bologna, Italy
Duration: 14 Jun 201518 Jun 2015
Conference number: 14
http://www.ishs.org/symposium/382

Conference

ConferenceEucarpia Symposium on Fruit Breeding and Genetics 2015
Number14
Country/TerritoryItaly
CityBologna
Period14/06/201518/06/2015
Internet address
SeriesActa Horticulturae
Volume1172
ISSN0567-7572

Keywords

  • Apples

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