Deep muscle-proteomic analysis of freeze-dried human muscle biopsies reveals fiber type-specific adaptations to exercise training

Atul Shahaji Deshmukh*, Dorte Enggaard Steenberg, Morten Hostrup, Jesper Bratz Birk, Jeppe Kjærgaard Larsen, A. Santos, Rasmus Kjøbsted, Janne Rasmuss Hingst, Camilla Charlotte Schéele, Marta Murgia, Bente Kiens, Erik A. Richter, Matthias Mann, Jørgen Wojtaszewski*

*Corresponding author for this work

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Abstract

Skeletal muscle conveys several of the health-promoting effects of exercise; yet the underlying mechanisms are not fully elucidated. Studying skeletal muscle is challenging due to its different fiber types and the presence of non-muscle cells. This can be circumvented by isolation of single muscle fibers. Here, we develop a workflow enabling proteomics analysis of pools of isolated muscle fibers from freeze-dried human muscle biopsies. We identify more than 4000 proteins in slow- and fast-twitch muscle fibers. Exercise training alters expression of 237 and 172 proteins in slow- and fast-twitch muscle fibers, respectively. Interestingly, expression levels of secreted proteins and proteins involved in transcription, mitochondrial metabolism, Ca2+ signaling, and fat and glucose metabolism adapts to training in a fiber type-specific manner. Our data provide a resource to elucidate molecular mechanisms underlying muscle function and health, and our workflow allows fiber type-specific proteomic analyses of snap-frozen non-embedded human muscle biopsies.

Original languageEnglish
Article number304
JournalNature Communications
Volume12
Number of pages15
ISSN2041-1723
DOIs
Publication statusPublished - 2021

Bibliographical note

A correction to this has been published at: https://doi.org/10.1038/s41467-021-22015-4

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