Design and validation of conditional ligands for HLA-B*08:01, HLA-B*15:01, HLA-B*35:01, and HLA-B*44:05

Thomas Mørch Frøsig; Jiawei Yap; Tina Seremet; Rikke Lyngaa; Inge Marie Svane; Per Thor Straten; Mirjam H M Heemskerk; Gijsbert M Grotenbreg; Sine Reker Hadrup

doi:10.1002/cyto.a.22689

Design and validation of conditional ligands for HLA-B08:01, HLA-B15:01, HLA-B35:01, and HLA-B44:05

Thomas Mørch Frøsig, Jiawei Yap, Tina Seremet, Rikke Lyngaa, Inge Marie Svane, Per Thor Straten, Mirjam H M Heemskerk, Gijsbert M Grotenbreg, Sine Reker Hadrup

Department of Clinical Medicine

Research output: Contribution to journal › Journal article › Research › peer-review

15 Citations (Scopus)

Abstract

We designed conditional ligands restricted to HLA-B*08:01, -B*35:01, and -B*44:05 and proved the use of a conditional ligand previously designed for HLA-B*15:02 together with HLA-B*15:01. Furthermore, we compared the detection capabilities of specific HLA-B*15:01-restricted T cells using the HLA-B*15:01 and HLA-B*15:02 major histocompatibility complex (MHC) multimers and found remarkable differences in the staining patterns detected by flow cytometry. These new conditional ligands greatly add to the application of MHC-based technologies in the analyses of T-cell recognition as they represent frequently expressed HLA-B molecules. This expansion of conditional ligands is important to allow T-cell detection over a wide range of HLA restrictions, and provide comprehensive understanding of the T-cell recognition in a given context.

Original language	English
Journal	Cytometry. Part A
Volume	87
Issue number	10
Pages (from-to)	967-75
Number of pages	9
ISSN	1552-4922
DOIs	https://doi.org/10.1002/cyto.a.22689
Publication status	Published - Oct 2015

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@article{6d1ee9704ff143ad899d4a51b6d72ebd,

title = "Design and validation of conditional ligands for HLA-B*08:01, HLA-B*15:01, HLA-B*35:01, and HLA-B*44:05",

abstract = "We designed conditional ligands restricted to HLA-B*08:01, -B*35:01, and -B*44:05 and proved the use of a conditional ligand previously designed for HLA-B*15:02 together with HLA-B*15:01. Furthermore, we compared the detection capabilities of specific HLA-B*15:01-restricted T cells using the HLA-B*15:01 and HLA-B*15:02 major histocompatibility complex (MHC) multimers and found remarkable differences in the staining patterns detected by flow cytometry. These new conditional ligands greatly add to the application of MHC-based technologies in the analyses of T-cell recognition as they represent frequently expressed HLA-B molecules. This expansion of conditional ligands is important to allow T-cell detection over a wide range of HLA restrictions, and provide comprehensive understanding of the T-cell recognition in a given context.",

author = "Fr{\o}sig, {Thomas M{\o}rch} and Jiawei Yap and Tina Seremet and Rikke Lyngaa and Svane, {Inge Marie} and {Thor Straten}, Per and Heemskerk, {Mirjam H M} and Grotenbreg, {Gijsbert M} and Hadrup, {Sine Reker}",

note = "{\textcopyright} 2015 International Society for Advancement of Cytometry.",

year = "2015",

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doi = "10.1002/cyto.a.22689",

language = "English",

volume = "87",

pages = "967--75",

journal = "Cytometry. Part A",

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T1 - Design and validation of conditional ligands for HLA-B*08:01, HLA-B*15:01, HLA-B*35:01, and HLA-B*44:05

AU - Frøsig, Thomas Mørch

AU - Yap, Jiawei

AU - Seremet, Tina

AU - Lyngaa, Rikke

AU - Svane, Inge Marie

AU - Thor Straten, Per

AU - Heemskerk, Mirjam H M

AU - Grotenbreg, Gijsbert M

AU - Hadrup, Sine Reker

PY - 2015/10

Y1 - 2015/10

N2 - We designed conditional ligands restricted to HLA-B*08:01, -B*35:01, and -B*44:05 and proved the use of a conditional ligand previously designed for HLA-B*15:02 together with HLA-B*15:01. Furthermore, we compared the detection capabilities of specific HLA-B*15:01-restricted T cells using the HLA-B*15:01 and HLA-B*15:02 major histocompatibility complex (MHC) multimers and found remarkable differences in the staining patterns detected by flow cytometry. These new conditional ligands greatly add to the application of MHC-based technologies in the analyses of T-cell recognition as they represent frequently expressed HLA-B molecules. This expansion of conditional ligands is important to allow T-cell detection over a wide range of HLA restrictions, and provide comprehensive understanding of the T-cell recognition in a given context.

AB - We designed conditional ligands restricted to HLA-B*08:01, -B*35:01, and -B*44:05 and proved the use of a conditional ligand previously designed for HLA-B*15:02 together with HLA-B*15:01. Furthermore, we compared the detection capabilities of specific HLA-B*15:01-restricted T cells using the HLA-B*15:01 and HLA-B*15:02 major histocompatibility complex (MHC) multimers and found remarkable differences in the staining patterns detected by flow cytometry. These new conditional ligands greatly add to the application of MHC-based technologies in the analyses of T-cell recognition as they represent frequently expressed HLA-B molecules. This expansion of conditional ligands is important to allow T-cell detection over a wide range of HLA restrictions, and provide comprehensive understanding of the T-cell recognition in a given context.

U2 - 10.1002/cyto.a.22689

DO - 10.1002/cyto.a.22689

M3 - Journal article

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SN - 1552-4922

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SP - 967

EP - 975

JO - Cytometry. Part A

JF - Cytometry. Part A

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