Determination of beta-defensin genomic copy number in different populations: a comparison of three methods

Peder Fode; Cathrine Jespersgaard; Robert J Hardwick; Helen Bogle; Michael Theisen; Daniel Dodoo; Martin Lenicek; Libor Vitek; Ana Vieira; Joao Freitas; Paal Skytt Andersen; Edward J Hollox

doi:10.1371/journal.pone.0016768

Determination of beta-defensin genomic copy number in different populations: a comparison of three methods

Peder Fode, Cathrine Jespersgaard, Robert J Hardwick, Helen Bogle, Michael Theisen, Daniel Dodoo, Martin Lenicek, Libor Vitek, Ana Vieira, Joao Freitas, Paal Skytt Andersen, Edward J Hollox

Research output: Contribution to journal › Journal article › Research › peer-review

41 Citations (Scopus)

Abstract

There have been conflicting reports in the literature on association of gene copy number with disease, including CCL3L1 and HIV susceptibility, and ß-defensins and Crohn's disease. Quantification of precise gene copy numbers is important in order to define any association of gene copy number with disease. At present, real-time quantitative PCR (QPCR) is the most commonly used method to determine gene copy number, however the Paralogue Ratio Test (PRT) is being used in more and more laboratories.

Original language	English
Journal	P L o S One
Volume	6
Issue number	2
Pages (from-to)	e16768
ISSN	1932-6203
DOIs	https://doi.org/10.1371/journal.pone.0016768
Publication status	Published - 2011

Keywords

Base Sequence
Chromosome Mapping
Cohort Studies
Czech Republic
Denmark
Gene Dosage
Genetic Predisposition to Disease
Genetics, Population
Genome, Human
Ghana
Great Britain
Humans
Molecular Sequence Data
Population
Portugal
beta-Defensins

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10.1371/journal.pone.0016768

Cite this

Determination of beta-defensin genomic copy number in different populations : a comparison of three methods. / Fode, Peder; Jespersgaard, Cathrine; Hardwick, Robert J; Bogle, Helen; Theisen, Michael; Dodoo, Daniel; Lenicek, Martin; Vitek, Libor; Vieira, Ana; Freitas, Joao; Andersen, Paal Skytt; Hollox, Edward J.

In: P L o S One, Vol. 6, No. 2, 2011, p. e16768.

Research output: Contribution to journal › Journal article › Research › peer-review

Fode, Peder ; Jespersgaard, Cathrine ; Hardwick, Robert J ; Bogle, Helen ; Theisen, Michael ; Dodoo, Daniel ; Lenicek, Martin ; Vitek, Libor ; Vieira, Ana ; Freitas, Joao ; Andersen, Paal Skytt ; Hollox, Edward J. / Determination of beta-defensin genomic copy number in different populations : a comparison of three methods. In: P L o S One. 2011 ; Vol. 6, No. 2. pp. e16768.

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