Development of a one-step probe based molecular assay for rapid immunodiagnosis of infection with M. Tuberculosisusing dried blood spots

Thomas Blauenfeldt; Jan Heyckendorf; Sidse Graff Jensen; Christoph Lange; Camilla Drabe; Thomas S. Hermansen; Lena De Thurah; Troels Lillebaek; Jesper Eugen-Olsen; Niels Seersholm; Søren Hoff; Jesper Bonde; Morten Ruhwald

doi:10.1371/journal.pone.0105628

Development of a one-step probe based molecular assay for rapid immunodiagnosis of infection with M. Tuberculosisusing dried blood spots

Thomas Blauenfeldt, Jan Heyckendorf, Sidse Graff Jensen, Christoph Lange, Camilla Drabe, Thomas S. Hermansen, Lena De Thurah, Troels Lillebaek, Jesper Eugen-Olsen, Niels Seersholm, Søren Hoff, Jesper Bonde, Morten Ruhwald

Research output: Contribution to journal › Journal article › Research › peer-review

22 Citations (Scopus)

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Abstract

Background: Antigen specific release of IP-10 is the most promising alternative marker to IFN-γ for infection with M. tuberculosis. Compared to Interferon-γ release assays (IGRA), IP-10 is released in high levels enabling novel approaches such as field friendly dried blood spots (DBS) and molecular detection.

Original language	English
Article number	e105628
Journal	PLoS ONE
Volume	9
Issue number	9
ISSN	1932-6203
DOIs	https://doi.org/10.1371/journal.pone.0105628
Publication status	Published - 3 Sept 2014
Externally published	Yes

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10.1371/journal.pone.0105628Licence: CC BY

Development of a one-step probe based molecular assay for rapid immunodiagnosis of infection with M. Tuberculosisusing dried blood spotsFinal published version, 542 KBLicence: CC BY

Cite this

Blauenfeldt, T., Heyckendorf, J., Jensen, S. G., Lange, C., Drabe, C., Hermansen, T. S., De Thurah, L., Lillebaek, T., Eugen-Olsen, J., Seersholm, N., Hoff, S., Bonde, J., & Ruhwald, M. (2014). Development of a one-step probe based molecular assay for rapid immunodiagnosis of infection with M. Tuberculosisusing dried blood spots. PLoS ONE, 9(9), Article e105628. https://doi.org/10.1371/journal.pone.0105628

Blauenfeldt, T, Heyckendorf, J, Jensen, SG, Lange, C, Drabe, C, Hermansen, TS, De Thurah, L, Lillebaek, T, Eugen-Olsen, J, Seersholm, N, Hoff, S, Bonde, J & Ruhwald, M 2014, 'Development of a one-step probe based molecular assay for rapid immunodiagnosis of infection with M. Tuberculosisusing dried blood spots', PLoS ONE, vol. 9, no. 9, e105628. https://doi.org/10.1371/journal.pone.0105628

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AU - Drabe, Camilla

AU - Hermansen, Thomas S.

AU - De Thurah, Lena

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AU - Eugen-Olsen, Jesper

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AU - Bonde, Jesper

AU - Ruhwald, Morten

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AB - Background: Antigen specific release of IP-10 is the most promising alternative marker to IFN-γ for infection with M. tuberculosis. Compared to Interferon-γ release assays (IGRA), IP-10 is released in high levels enabling novel approaches such as field friendly dried blood spots (DBS) and molecular detection.

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