Development of Volatile Compounds during Hydrolysis of Porcine Hemoglobin with Papain

Kathrine Holmgaard Bak, Mikael Agerlin Petersen, Rene Lametsch, Erik T. Hansen, Jorge Ruiz Carrascal

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Abstract

There is a growing market for the use of hydrolysates from animal side-streams for production of high-protein supplements. However, there can be issues with development of off-flavors, either due to the raw material in question or due to the hydrolysis process itself. This study examined the development of volatile compounds during hydrolysis of hemoglobin. Briefly, porcine hemoglobin was hydrolyzed by 0.5% papain for up to 5 h, and the development of volatile compounds was analyzed via gas chromatography-mass spectrometry. The results showed that there was significant development of a number of volatile compounds with time, e.g., certain Maillard reaction and lipid oxidation products, which are likely candidates for the aroma development during hydrolysis. Furthermore, it was shown that development of a number of the volatiles was due to the hydrolysis process, as these compounds were not found in a control without enzyme.
Original languageEnglish
Article number357
JournalMolecules
Volume23
Issue number2
Number of pages9
ISSN1420-3049
DOIs
Publication statusPublished - 8 Feb 2018

Keywords

  • hydrolysis
  • enzyme
  • enzymatic hydrolysis
  • hemoglobin
  • papain
  • aroma
  • volatile compounds

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