Direct Electromembrane Extraction‐Based Mass Spectrometry: A Tool for Studying Drug Metabolism Properties of Liver Organoids

Frøydis Sved Skottvoll; Aleksandra Aizenshtadt; Frederik André Hansen; Mikel Amirola Martinez; Jannike Mørch Andersen; Inger Lise Bogen; Jörg P. Kutter; Stig Pedersen‐bjergaard; Elsa Lundanes; Stefan Krauss; Steven Ray Wilson

doi:10.1002/anse.202100051

Direct Electromembrane Extraction‐Based Mass Spectrometry: A Tool for Studying Drug Metabolism Properties of Liver Organoids

Frøydis Sved Skottvoll, Aleksandra Aizenshtadt, Frederik André Hansen, Mikel Amirola Martinez, Jannike Mørch Andersen, Inger Lise Bogen, Jörg P. Kutter, Stig Pedersen‐bjergaard, Elsa Lundanes, Stefan Krauss, Steven Ray Wilson

Microscale Analytical Systems

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

This work introduces a strategy for organoid analysis - direct Electromembrane Extraction based Mass Spectrometry (dEME-MS) – for coupling liver organoids with mass spectrometry (MS). dEME-MS comprises electrophoresis of selected small molecules from a culture chamber across an oil membrane, and to a MS compatible solution. This enables clean micro-extraction of drugs and their metabolites as produced in the liver organoids to capillary liquid chromatography-mass spectrometry. Applying dEME-MS, proof-of-concept of directly measuring methadone metabolism is demonstrated on adult liver organoids. With 50 liver organoids and 1 μM methadone, methadone metabolism was monitored from 0 to 24 hours (11 time points). All analytes had <0.4 % variance in retention times with >100 measurements. dEME-MS is capable of automated and selective monitoring of drug metabolism in liver organoids, and could serve as a valuable tool for automated drug discovery efforts.

Original language	English
Article number	e202100051
Journal	Analysis & Sensing
Volume	2
Issue number	2
ISSN	2629-2742
DOIs	https://doi.org/10.1002/anse.202100051
Publication status	Published - 2022

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Skottvoll, F. S., Aizenshtadt, A., Hansen, F. A., Martinez, M. A., Andersen, J. M., Bogen, I. L., Kutter, J. P., Pedersen‐bjergaard, S., Lundanes, E., Krauss, S., & Wilson, S. R. (2022). Direct Electromembrane Extraction‐Based Mass Spectrometry: A Tool for Studying Drug Metabolism Properties of Liver Organoids. Analysis & Sensing, 2(2), [e202100051]. https://doi.org/10.1002/anse.202100051

Direct Electromembrane Extraction‐Based Mass Spectrometry: A Tool for Studying Drug Metabolism Properties of Liver Organoids. / Skottvoll, Frøydis Sved; Aizenshtadt, Aleksandra; Hansen, Frederik André; Martinez, Mikel Amirola; Andersen, Jannike Mørch; Bogen, Inger Lise; Kutter, Jörg P.; Pedersen‐bjergaard, Stig; Lundanes, Elsa; Krauss, Stefan; Wilson, Steven Ray.

In: Analysis & Sensing, Vol. 2, No. 2, e202100051, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Skottvoll, FS, Aizenshtadt, A, Hansen, FA, Martinez, MA, Andersen, JM, Bogen, IL, Kutter, JP , Pedersen‐bjergaard, S, Lundanes, E, Krauss, S & Wilson, SR 2022, 'Direct Electromembrane Extraction‐Based Mass Spectrometry: A Tool for Studying Drug Metabolism Properties of Liver Organoids', Analysis & Sensing, vol. 2, no. 2, e202100051. https://doi.org/10.1002/anse.202100051

Skottvoll, Frøydis Sved ; Aizenshtadt, Aleksandra ; Hansen, Frederik André ; Martinez, Mikel Amirola ; Andersen, Jannike Mørch ; Bogen, Inger Lise ; Kutter, Jörg P. ; Pedersen‐bjergaard, Stig ; Lundanes, Elsa ; Krauss, Stefan ; Wilson, Steven Ray. / Direct Electromembrane Extraction‐Based Mass Spectrometry: A Tool for Studying Drug Metabolism Properties of Liver Organoids. In: Analysis & Sensing. 2022 ; Vol. 2, No. 2.

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abstract = "This work introduces a strategy for organoid analysis - direct Electromembrane Extraction based Mass Spectrometry (dEME-MS) – for coupling liver organoids with mass spectrometry (MS). dEME-MS comprises electrophoresis of selected small molecules from a culture chamber across an oil membrane, and to a MS compatible solution. This enables clean micro-extraction of drugs and their metabolites as produced in the liver organoids to capillary liquid chromatography-mass spectrometry. Applying dEME-MS, proof-of-concept of directly measuring methadone metabolism is demonstrated on adult liver organoids. With 50 liver organoids and 1 μM methadone, methadone metabolism was monitored from 0 to 24 hours (11 time points). All analytes had <0.4 % variance in retention times with >100 measurements. dEME-MS is capable of automated and selective monitoring of drug metabolism in liver organoids, and could serve as a valuable tool for automated drug discovery efforts.",

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AU - Skottvoll, Frøydis Sved

AU - Aizenshtadt, Aleksandra

AU - Hansen, Frederik André

AU - Martinez, Mikel Amirola

AU - Andersen, Jannike Mørch

AU - Bogen, Inger Lise

AU - Kutter, Jörg P.

AU - Pedersen‐bjergaard, Stig

AU - Lundanes, Elsa

AU - Krauss, Stefan

AU - Wilson, Steven Ray

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AB - This work introduces a strategy for organoid analysis - direct Electromembrane Extraction based Mass Spectrometry (dEME-MS) – for coupling liver organoids with mass spectrometry (MS). dEME-MS comprises electrophoresis of selected small molecules from a culture chamber across an oil membrane, and to a MS compatible solution. This enables clean micro-extraction of drugs and their metabolites as produced in the liver organoids to capillary liquid chromatography-mass spectrometry. Applying dEME-MS, proof-of-concept of directly measuring methadone metabolism is demonstrated on adult liver organoids. With 50 liver organoids and 1 μM methadone, methadone metabolism was monitored from 0 to 24 hours (11 time points). All analytes had <0.4 % variance in retention times with >100 measurements. dEME-MS is capable of automated and selective monitoring of drug metabolism in liver organoids, and could serve as a valuable tool for automated drug discovery efforts.

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