Direct Observation of CRISPR-Cas12 Conformational Sampling by SM FRET and Cryo EM Reveals how Conformational Activation Promotes Catalysis and Resetting of the Endonuclease Activity

Stefano Stella; Pablo Mesa; Johannes Thomsen; Bijoya Paul; Pablo Alcon; Simon B. Jensen; Matias E. Moses; Guillermo Montoya; Nikos S. Hatzakis

doi:10.1016/j.bpj.2019.11.1324

Direct Observation of CRISPR-Cas12 Conformational Sampling by SM FRET and Cryo EM Reveals how Conformational Activation Promotes Catalysis and Resetting of the Endonuclease Activity

Stefano Stella, Pablo Mesa, Johannes Thomsen, Bijoya Paul, Pablo Alcon, Simon B. Jensen, Matias E. Moses, Guillermo Montoya, Nikos S. Hatzakis

Research output: Contribution to journal › Conference abstract in journal › Research

Original language	English
Journal	Biophysical Journal
Volume	118
Issue number	3
Pages (from-to)	223A-224A
Number of pages	2
ISSN	0006-3495
DOIs	https://doi.org/10.1016/j.bpj.2019.11.1324
Publication status	Published - 7 Feb 2020

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10.1016/j.bpj.2019.11.1324

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Stella, S., Mesa, P., Thomsen, J., Paul, B., Alcon, P., Jensen, S. B., Moses, M. E., Montoya, G., & Hatzakis, N. S. (2020). Direct Observation of CRISPR-Cas12 Conformational Sampling by SM FRET and Cryo EM Reveals how Conformational Activation Promotes Catalysis and Resetting of the Endonuclease Activity. Biophysical Journal, 118(3), 223A-224A. https://doi.org/10.1016/j.bpj.2019.11.1324

Direct Observation of CRISPR-Cas12 Conformational Sampling by SM FRET and Cryo EM Reveals how Conformational Activation Promotes Catalysis and Resetting of the Endonuclease Activity. / Stella, Stefano; Mesa, Pablo; Thomsen, Johannes; Paul, Bijoya; Alcon, Pablo; Jensen, Simon B.; Moses, Matias E.; Montoya, Guillermo ; Hatzakis, Nikos S.

In: Biophysical Journal, Vol. 118, No. 3, 07.02.2020, p. 223A-224A.

Research output: Contribution to journal › Conference abstract in journal › Research

Stella, S, Mesa, P, Thomsen, J, Paul, B, Alcon, P, Jensen, SB, Moses, ME, Montoya, G & Hatzakis, NS 2020, 'Direct Observation of CRISPR-Cas12 Conformational Sampling by SM FRET and Cryo EM Reveals how Conformational Activation Promotes Catalysis and Resetting of the Endonuclease Activity', Biophysical Journal, vol. 118, no. 3, pp. 223A-224A. https://doi.org/10.1016/j.bpj.2019.11.1324

Stella, Stefano ; Mesa, Pablo ; Thomsen, Johannes ; Paul, Bijoya ; Alcon, Pablo ; Jensen, Simon B. ; Moses, Matias E. ; Montoya, Guillermo ; Hatzakis, Nikos S. / Direct Observation of CRISPR-Cas12 Conformational Sampling by SM FRET and Cryo EM Reveals how Conformational Activation Promotes Catalysis and Resetting of the Endonuclease Activity. In: Biophysical Journal. 2020 ; Vol. 118, No. 3. pp. 223A-224A.

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