Directly from the source: endogenous preparations of molecular machines

Pablo Mesa; Aurélien Deniaud; Guillermo Montoya; Christiane Schaffitzel

doi:10.1016/j.sbi.2013.01.005

Directly from the source: endogenous preparations of molecular machines

Pablo Mesa, Aurélien Deniaud, Guillermo Montoya, Christiane Schaffitzel

Research output: Contribution to journal › Review › Research › peer-review

12 Citations (Scopus)

Abstract

Purification from a source enriched in large macromolecular machines with basic cellular function is still the method of choice in many cases. Such complexes occur in sufficiently high copy numbers in the cell and can be isolated using classical protein purification protocols. Although advanced DNA recombinant technologies and sophisticated overexpression strategies are available, many complexes like the ribosome, RNA polymerase II and membrane protein complexes involved in photosynthesis or in oxidative phosphorylation can only be purified from a rich source. Here, we review recent accomplishments and limitations in applying this strategy.

Original language	English
Journal	Current Opinion in Structural Biology
Volume	23
Issue number	3
Pages (from-to)	319-25
Number of pages	7
ISSN	0959-440X
DOIs	https://doi.org/10.1016/j.sbi.2013.01.005
Publication status	Published - Jun 2013
Externally published	Yes

Bibliographical note

Keywords

Analytic Sample Preparation Methods
Animals
Bacteria/chemistry
Crystallization
DNA, Recombinant/genetics
Databases, Protein
Humans
Multiprotein Complexes/chemistry
Protein Conformation
Recombinant Proteins/biosynthesis
Yeasts/chemistry

Access to Document

10.1016/j.sbi.2013.01.005

Cite this

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title = "Directly from the source: endogenous preparations of molecular machines",

abstract = "Purification from a source enriched in large macromolecular machines with basic cellular function is still the method of choice in many cases. Such complexes occur in sufficiently high copy numbers in the cell and can be isolated using classical protein purification protocols. Although advanced DNA recombinant technologies and sophisticated overexpression strategies are available, many complexes like the ribosome, RNA polymerase II and membrane protein complexes involved in photosynthesis or in oxidative phosphorylation can only be purified from a rich source. Here, we review recent accomplishments and limitations in applying this strategy.",

keywords = "Analytic Sample Preparation Methods, Animals, Bacteria/chemistry, Crystallization, DNA, Recombinant/genetics, Databases, Protein, Humans, Multiprotein Complexes/chemistry, Protein Conformation, Recombinant Proteins/biosynthesis, Yeasts/chemistry",

author = "Pablo Mesa and Aur{\'e}lien Deniaud and Guillermo Montoya and Christiane Schaffitzel",

note = "Copyright {\textcopyright} 2013. Published by Elsevier Ltd.",

year = "2013",

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doi = "10.1016/j.sbi.2013.01.005",

language = "English",

volume = "23",

pages = "319--25",

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T2 - endogenous preparations of molecular machines

AU - Mesa, Pablo

AU - Deniaud, Aurélien

AU - Montoya, Guillermo

AU - Schaffitzel, Christiane

PY - 2013/6

Y1 - 2013/6

N2 - Purification from a source enriched in large macromolecular machines with basic cellular function is still the method of choice in many cases. Such complexes occur in sufficiently high copy numbers in the cell and can be isolated using classical protein purification protocols. Although advanced DNA recombinant technologies and sophisticated overexpression strategies are available, many complexes like the ribosome, RNA polymerase II and membrane protein complexes involved in photosynthesis or in oxidative phosphorylation can only be purified from a rich source. Here, we review recent accomplishments and limitations in applying this strategy.

AB - Purification from a source enriched in large macromolecular machines with basic cellular function is still the method of choice in many cases. Such complexes occur in sufficiently high copy numbers in the cell and can be isolated using classical protein purification protocols. Although advanced DNA recombinant technologies and sophisticated overexpression strategies are available, many complexes like the ribosome, RNA polymerase II and membrane protein complexes involved in photosynthesis or in oxidative phosphorylation can only be purified from a rich source. Here, we review recent accomplishments and limitations in applying this strategy.

KW - Analytic Sample Preparation Methods

KW - Animals

KW - Bacteria/chemistry

KW - Crystallization

KW - DNA, Recombinant/genetics

KW - Databases, Protein

KW - Humans

KW - Multiprotein Complexes/chemistry

KW - Protein Conformation

KW - Recombinant Proteins/biosynthesis

KW - Yeasts/chemistry

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DO - 10.1016/j.sbi.2013.01.005

M3 - Review

C2 - 23395510

VL - 23

SP - 319

EP - 325

JO - Current Opinion in Structural Biology

JF - Current Opinion in Structural Biology

SN - 0959-440X

IS - 3

ER -