TY - JOUR
T1 - Distinct galactofuranose antigens in the cell wall and culture supernatants as a means to differentiate Fusarium from AspergillusspeciesAnnegret
AU - Wiedemann, Annegret
AU - Kakoschke, Tamara Katharina
AU - Speth, Cornelia
AU - Rambach, Günter
AU - Ensinger, Christian
AU - Jensen, Henrik Elvang
AU - Ebel, Frank
PY - 2016
Y1 - 2016
N2 - tDetection of carbohydrate antigens is an important means for diagnosis of invasive fungal infections. Fordiagnosis of systemic Aspergillus infections, galactomannan is commonly used, the core antigenic struc-ture of which consists of chains of several galactofuranose moieties. In this study, we provide evidence thatFusarium produces at least two distinct galactofuranose antigens: Smaller amounts of galactomannanand larger quantities of a novel antigen recognized by the monoclonal antibody AB135-8. In A. fumiga-tus, only minor amounts of the AB135-8 antigen are found in supernatants and in the apical regions ofhyphae. A galactofuranose-deficient A. fumigatus mutant lacks the AB135-8 antigen, which strongly sug-gests that galactofuranose is an essential constituent of this antigen. Using a combination of AB135-8 anda galactomannan-specific antibody, we were able to unambiguously differentiate A. fumigatus and Fusar-ium hyphae in immunohistology. Moreover, since Fusarium releases the AB135-8 antigen, it appears tobe a promising target antigen for a serological detection of Fusarium infections.
AB - tDetection of carbohydrate antigens is an important means for diagnosis of invasive fungal infections. Fordiagnosis of systemic Aspergillus infections, galactomannan is commonly used, the core antigenic struc-ture of which consists of chains of several galactofuranose moieties. In this study, we provide evidence thatFusarium produces at least two distinct galactofuranose antigens: Smaller amounts of galactomannanand larger quantities of a novel antigen recognized by the monoclonal antibody AB135-8. In A. fumiga-tus, only minor amounts of the AB135-8 antigen are found in supernatants and in the apical regions ofhyphae. A galactofuranose-deficient A. fumigatus mutant lacks the AB135-8 antigen, which strongly sug-gests that galactofuranose is an essential constituent of this antigen. Using a combination of AB135-8 anda galactomannan-specific antibody, we were able to unambiguously differentiate A. fumigatus and Fusar-ium hyphae in immunohistology. Moreover, since Fusarium releases the AB135-8 antigen, it appears tobe a promising target antigen for a serological detection of Fusarium infections.
U2 - 10.1016/j.ijmm.2016.05.002
DO - 10.1016/j.ijmm.2016.05.002
M3 - Journal article
C2 - 27237422
VL - 306
SP - 381
EP - 390
JO - International Journal of Medical Microbiology
JF - International Journal of Medical Microbiology
SN - 1438-4221
ER -