TY - JOUR
T1 - Distribution patterns of postmortem damage in human mitochondrial DNA
AU - Gilbert, M Thomas P
AU - Willerslev, Eske
AU - Hansen, Anders J
AU - Barnes, Ian
AU - Rudbeck, Lars
AU - Lynnerup, Niels
AU - Cooper, Alan
N1 - Keywords: Animals; DNA Damage; DNA Glycosylases; DNA, Mitochondrial; Electron Transport Complex IV; Haplotypes; Humans; Mitochondria; Mitochondrial Proteins; Mutagenesis; N-Glycosyl Hydrolases; Polymerase Chain Reaction; Postmortem Changes; Species Specificity; Tooth; Uracil-DNA Glycosidase; Ursidae
PY - 2002
Y1 - 2002
N2 - The distribution of postmortem damage in mitochondrial DNA retrieved from 37 ancient human DNA samples was analyzed by cloning and was compared with a selection of published animal data. A relative rate of damage (rho(v)) was calculated for nucleotide positions within the human hypervariable region 1 (HVR1) and cytochrome oxidase subunit III genes. A comparison of damaged sites within and between the regions reveals that damage hotspots exist and that, in the HVR1, these correlate with sites known to have high in vivo mutation rates. Conversely, HVR1 subregions with known structural function, such as MT5, have lower in vivo mutation rates and lower postmortem-damage rates. The postmortem data also identify a possible functional subregion of the HVR1, termed "low-diversity 1," through the lack of sequence damage. The amount of postmortem damage observed in mitochondrial coding regions was significantly lower than in the HVR1, and, although hotspots were noted, these did not correlate with codon position. Finally, a simple method for the identification of incorrect archaeological haplogroup designations is introduced, on the basis of the observed spectrum of postmortem damage.
AB - The distribution of postmortem damage in mitochondrial DNA retrieved from 37 ancient human DNA samples was analyzed by cloning and was compared with a selection of published animal data. A relative rate of damage (rho(v)) was calculated for nucleotide positions within the human hypervariable region 1 (HVR1) and cytochrome oxidase subunit III genes. A comparison of damaged sites within and between the regions reveals that damage hotspots exist and that, in the HVR1, these correlate with sites known to have high in vivo mutation rates. Conversely, HVR1 subregions with known structural function, such as MT5, have lower in vivo mutation rates and lower postmortem-damage rates. The postmortem data also identify a possible functional subregion of the HVR1, termed "low-diversity 1," through the lack of sequence damage. The amount of postmortem damage observed in mitochondrial coding regions was significantly lower than in the HVR1, and, although hotspots were noted, these did not correlate with codon position. Finally, a simple method for the identification of incorrect archaeological haplogroup designations is introduced, on the basis of the observed spectrum of postmortem damage.
U2 - 10.1086/345378
DO - 10.1086/345378
M3 - Journal article
C2 - 12489041
VL - 72
SP - 32
EP - 47
JO - American Journal of Human Genetics
JF - American Journal of Human Genetics
SN - 0002-9297
IS - 1
ER -