DNA-AgNC Loaded Liposomes for Measuring Cerebral Blood Flow Using Two-Photon Fluorescence Correlation Spectroscopy

Xiaojin Wang; Mikkel B. Liisberg; Georgia L. Nolt; Xu Fu; Cecilia Cerretani; Lan Li; Lance A. Johnson; Tom Vosch; Christopher I. Richards

doi:10.1021/acsnano.3c04489

DNA-AgNC Loaded Liposomes for Measuring Cerebral Blood Flow Using Two-Photon Fluorescence Correlation Spectroscopy

Xiaojin Wang, Mikkel B. Liisberg, Georgia L. Nolt, Xu Fu, Cecilia Cerretani, Lan Li, Lance A. Johnson, Tom Vosch, Christopher I. Richards

Department of Chemistry

Research output: Contribution to journal › Journal article › Research › peer-review

9 Citations (Scopus)

3 Downloads (Pure)

Abstract

Unraveling the transport of drugs and nanocarriers in cerebrovascular networks is important for pharmacokinetic and hemodynamic studies but is challenging due to the complexity of sensing individual particles within the circulatory system of a live animal. Here, we demonstrate that a DNA-stabilized silver nanocluster (DNA-Ag16NC) that emits in the first near-infrared window upon two-photon excitation in the second NIR window can be used for multiphoton in vivo fluorescence correlation spectroscopy for the measurement of cerebral blood flow rates in live mice with high spatial and temporal resolution. To ensure bright and stable emission during in vivo experiments, we loaded DNA-Ag16NCs into liposomes, which served the dual purposes of concentrating the fluorescent label and protecting it from degradation. DNA-Ag16NC-loaded liposomes enabled the quantification of cerebral blood flow velocities within individual vessels of a living mouse.

Original language	English
Journal	ACS Nano
Volume	17
Issue number	13
Pages (from-to)	12862–12874
Number of pages	13
ISSN	1936-0851
DOIs	https://doi.org/10.1021/acsnano.3c04489
Publication status	Published - 2023

Access to Document

10.1021/acsnano.3c04489

FulltextAccepted author manuscript, 2.4 MBLicence: Unspecified

Cite this

DNA-AgNC Loaded Liposomes for Measuring Cerebral Blood Flow Using Two-Photon Fluorescence Correlation Spectroscopy. / Wang, Xiaojin; Liisberg, Mikkel B.; Nolt, Georgia L.; Fu, Xu; Cerretani, Cecilia; Li, Lan; Johnson, Lance A.; Vosch, Tom; Richards, Christopher I.

In: ACS Nano, Vol. 17, No. 13, 2023, p. 12862–12874.

Research output: Contribution to journal › Journal article › Research › peer-review

@article{2c9eabaabecf4f0285a67c5eb0999bb1,

title = "DNA-AgNC Loaded Liposomes for Measuring Cerebral Blood Flow Using Two-Photon Fluorescence Correlation Spectroscopy",

abstract = "Unraveling the transport of drugs and nanocarriers in cerebrovascular networks is important for pharmacokinetic and hemodynamic studies but is challenging due to the complexity of sensing individual particles within the circulatory system of a live animal. Here, we demonstrate that a DNA-stabilized silver nanocluster (DNA-Ag16NC) that emits in the first near-infrared window upon two-photon excitation in the second NIR window can be used for multiphoton in vivo fluorescence correlation spectroscopy for the measurement of cerebral blood flow rates in live mice with high spatial and temporal resolution. To ensure bright and stable emission during in vivo experiments, we loaded DNA-Ag16NCs into liposomes, which served the dual purposes of concentrating the fluorescent label and protecting it from degradation. DNA-Ag16NC-loaded liposomes enabled the quantification of cerebral blood flow velocities within individual vessels of a living mouse.",

author = "Xiaojin Wang and Liisberg, {Mikkel B.} and Nolt, {Georgia L.} and Xu Fu and Cecilia Cerretani and Lan Li and Johnson, {Lance A.} and Tom Vosch and Richards, {Christopher I.}",

year = "2023",

doi = "10.1021/acsnano.3c04489",

language = "English",

volume = "17",

pages = "12862–12874",

journal = "A C S Nano",

issn = "1936-0851",

publisher = "American Chemical Society",

number = "13",

}

TY - JOUR

T1 - DNA-AgNC Loaded Liposomes for Measuring Cerebral Blood Flow Using Two-Photon Fluorescence Correlation Spectroscopy

AU - Wang, Xiaojin

AU - Liisberg, Mikkel B.

AU - Nolt, Georgia L.

AU - Fu, Xu

AU - Cerretani, Cecilia

AU - Li, Lan

AU - Johnson, Lance A.

AU - Vosch, Tom

AU - Richards, Christopher I.

PY - 2023

Y1 - 2023

N2 - Unraveling the transport of drugs and nanocarriers in cerebrovascular networks is important for pharmacokinetic and hemodynamic studies but is challenging due to the complexity of sensing individual particles within the circulatory system of a live animal. Here, we demonstrate that a DNA-stabilized silver nanocluster (DNA-Ag16NC) that emits in the first near-infrared window upon two-photon excitation in the second NIR window can be used for multiphoton in vivo fluorescence correlation spectroscopy for the measurement of cerebral blood flow rates in live mice with high spatial and temporal resolution. To ensure bright and stable emission during in vivo experiments, we loaded DNA-Ag16NCs into liposomes, which served the dual purposes of concentrating the fluorescent label and protecting it from degradation. DNA-Ag16NC-loaded liposomes enabled the quantification of cerebral blood flow velocities within individual vessels of a living mouse.

AB - Unraveling the transport of drugs and nanocarriers in cerebrovascular networks is important for pharmacokinetic and hemodynamic studies but is challenging due to the complexity of sensing individual particles within the circulatory system of a live animal. Here, we demonstrate that a DNA-stabilized silver nanocluster (DNA-Ag16NC) that emits in the first near-infrared window upon two-photon excitation in the second NIR window can be used for multiphoton in vivo fluorescence correlation spectroscopy for the measurement of cerebral blood flow rates in live mice with high spatial and temporal resolution. To ensure bright and stable emission during in vivo experiments, we loaded DNA-Ag16NCs into liposomes, which served the dual purposes of concentrating the fluorescent label and protecting it from degradation. DNA-Ag16NC-loaded liposomes enabled the quantification of cerebral blood flow velocities within individual vessels of a living mouse.

U2 - 10.1021/acsnano.3c04489

DO - 10.1021/acsnano.3c04489

M3 - Journal article

C2 - 37341451

VL - 17

SP - 12862

EP - 12874

JO - A C S Nano

JF - A C S Nano

SN - 1936-0851

IS - 13

ER -