DNA damage invokes mitophagy through a pathway involving Spata18

Xiuli Dan, Mansi Babbar, Anthony Moore, Noah Wechter, Jingyan Tian, Joy G Mohanty, Deborah L Croteau, Vilhelm A Bohr

Research output: Contribution to journalJournal articleResearchpeer-review

29 Citations (Scopus)

Abstract

Mitochondria are vital for cellular energy supply and intracellular signaling after stress. Here, we aimed to investigate how mitochondria respond to acute DNA damage with respect to mitophagy, which is an important mitochondrial quality control process. Our results show that mitophagy increases after DNA damage in primary fibroblasts, murine neurons and Caenorhabditis elegans neurons. Our results indicate that modulation of mitophagy after DNA damage is independent of the type of DNA damage stimuli used and that the protein Spata18 is an important player in this process. Knockdown of Spata18 suppresses mitophagy, disturbs mitochondrial Ca2+ homeostasis, affects ATP production, and attenuates DNA repair. Importantly, mitophagy after DNA damage is a vital cellular response to maintain mitochondrial functions and DNA repair.

Original languageEnglish
JournalNucleic Acids Research
Volume48
Issue number12
Pages (from-to)6611-6623
Number of pages13
ISSN0305-1048
DOIs
Publication statusPublished - 2020

Keywords

  • Animals
  • Caenorhabditis elegans/genetics
  • Calcium/metabolism
  • Cell Line
  • Cell Proliferation/genetics
  • DNA Damage/genetics
  • DNA Repair/genetics
  • Fibroblasts/metabolism
  • Humans
  • Mice
  • Mitochondria/genetics
  • Mitochondrial Proteins/genetics
  • Mitophagy/genetics
  • Neurons/metabolism

Cite this