Eccentric contractions affect muscle membrane phospholipid fatty acid composition in rats

Jørn Wulff Helge, K J Therkildsen, T B Jørgensen, B J Wu, L H Storlien, Stefan Erik Asp

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13 Citations (Scopus)

Abstract

This study investigated if prior eccentric contractions, and thus mechanical strain and muscle damage, exert an effect on the muscle membrane phospholipid fatty acid composition in rats, and whether a possible effect could be attenuated by dietary supplements. Twenty-three rats were randomised to three groups who received chow with added fish oil (n = 8), vitamin C (n = 8) or no supplement (n = 7). After 3 weeks of feeding, calf muscles on one side were stimulated electrically during anaesthesia causing eccentric contractions. Two days later the white gastrocnemius, a part of the stimulated calf muscle, was excised from both legs. In the muscles stimulated to contract eccentrically, compared to the control muscles, the proportion of arachidonic acid, C20:4,n-6 (17.7 +/- 0.6; 16.4 +/- 0.4% of total fatty acids, respectively) and docosapentanoeic acid, C22:5,n-3 (2.9 +/- 0.1 and 2.7 +/- 0.1% of total fatty acids, respectively) was uniformly higher across groups (P <0.02) with no differences between diet groups. The proportion of long chain polyunsaturates was also significantly higher in the eccentrically contracted (39.9 +/- 0.6% of total fatty acids) compared to the control leg (38.2 +/- 0.6% of total fatty acids; P <0.01). In contrast no differences were observed in the fatty acid composition of the triacylglycerols stored within the muscle. Thus one severe bout of eccentric contractions modulates the fatty acid composition of the muscle membrane phospholipids when compared to a control leg, and supplemental intake of fish oil or vitamin C did not attenuate this effect.
Original languageEnglish
JournalExperimental Physiology
Volume86
Issue number5
Pages (from-to)599-604
Number of pages6
ISSN0958-0670
Publication statusPublished - 1 Sep 2001

Keywords

  • Animals
  • Antioxidants
  • Ascorbic Acid
  • Blood Glucose
  • Dietary Fats
  • Electric Stimulation
  • Fatty Acids
  • Fish Oils
  • Lactic Acid
  • Male
  • Muscle Contraction
  • Muscle, Skeletal
  • Phospholipids
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Stress, Mechanical
  • Triglycerides

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