Effect of long- and short-term exposure to laser light at 1070 nm on growth of Saccharomyces cerevisiae

Thomas Ask Aabo, Ivan R. Perch-Nielsen, Jeppe Seidelin Dam, Darwin Palima, Henrik Siegumfeldt, Jesper Glückstad, Nils Arneborg

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    Abstract

    The effect of a 1070-nm continuous and pulsed wave ytterbium
    fiber laser on the growth of Saccharomyces cerevisiae single
    cells is investigated over a time span of 4 to 5 h. The cells are subjected
    to optical traps consisting of two counterpropagating plane
    wave beams with a uniform flux along the x, y axis. Even at the lowest
    continuous power investigated—i.e., 0.7 mW—the growth of S. cerevisiae
    cell clusters is markedly inhibited. The minimum power required
    to successfully trap single S. cerevisiae cells in three dimensions
    is estimated to be 3.5 mW. No threshold power for the
    photodamage, but instead a continuous response to the increased accumulated
    dose is found in the regime investigated from
    0.7 to 2.6 mW. Furthermore, by keeping the delivered dose constant
    and varying the exposure time and power—i.e. pulsing—we find that
    the growth of S. cerevisiae cells is increasingly inhibited with increasing
    power. These results indicate that growth of S. cerevisiae is dependent
    on both the power as well as the accumulated dose at
    1070 nm.

    Original languageEnglish
    JournalJournal of Biomedical Optics
    Volume15
    Issue number4
    Number of pages7
    ISSN1083-3668
    DOIs
    Publication statusPublished - 2010

    Keywords

    • Former LIFE faculty

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