Effect of sprint cycle training on activities of antioxidant enzymes in human skeletal muscle

Ylva Hellsten, F. S. Apple, B. Sjödin

    Research output: Contribution to journalJournal articleResearchpeer-review

    99 Citations (Scopus)

    Abstract

    The effect of intermittent sprint cycle training on the level of muscle antioxidant enzyme protection was investigated. Resting muscle biopsies, obtained before and after 6 wk of training and 3, 24, and 72 h after the final session of an additional 1 wk of more frequent training, were analyzed for activities of the antioxidant enzymes glutathione peroxidase (GPX), glutathione reductase (GR), and superoxide dismutase (SOD). Activities of several muscle metabolic enzymes were determined to assess the effectiveness of the training. After the first 6-wk training period, no change in GPX, GR, or SOD was observed, but after the 7th week of training there was an increase in GPX from 120 +/- 12 (SE) to 164 +/- 24 mumol.min-1.g dry wt-1 (P < 0.05) and in GR from 10.8 +/- 0.8 to 16.8 +/- 2.4 mumol.min-1.g dry wt-1 (P < 0.05). There was no significant change in SOD. Sprint cycle training induced a significant (P < 0.05) elevation in the activity of phosphofructokinase and creatine kinase, implying an enhanced anaerobic capacity in the trained muscle. The present study demonstrates that intermittent sprint cycle training that induces an enhanced capacity for anaerobic energy generation also improves the level of antioxidant protection in the muscle.
    Original languageEnglish
    JournalJournal of Applied Physiology
    Volume81
    Issue number4
    Pages (from-to)1484-1487
    Number of pages4
    ISSN8750-7587
    Publication statusPublished - 1996

    Bibliographical note

    Keywords: Adult; Anaerobic Threshold; Antioxidants; Bicycling; Glutathione Peroxidase; Glutathione Reductase; Humans; Male; Muscle, Skeletal; Physical Fitness; Superoxide Dismutase

    Cite this