Effects of F/G-actin ratio and actin turn-over rate on NADPH oxidase activity in microglia

Izabela Rasmussen; Line Hjortshøj Pedersen; Luise Byg; Kazuhiro Suzuki; Hideki Sumimoto; Frederik Vilhardt

doi:10.1186/1471-2172-11-44

Effects of F/G-actin ratio and actin turn-over rate on NADPH oxidase activity in microglia

Izabela Rasmussen, Line Hjortshøj Pedersen, Luise Byg, Kazuhiro Suzuki, Hideki Sumimoto, Frederik Vilhardt

Department of Cellular and Molecular Medicine

Research output: Contribution to journal › Journal article › Research › peer-review

48 Citations (Scopus)

Abstract

Most in vivo studies that have addressed the role of actin dynamics in NADPH oxidase function in phagocytes have used toxins to modulate the polymerization state of actin and mostly effects on actin has been evaluated by end point measurements of filamentous actin, which says little about actin dynamics, and without consideration for the subcellular distribution of the perturbed actin cytoskeleton.

Original language	English
Journal	B M C Immunology
Volume	11
Pages (from-to)	44
ISSN	1471-2172
DOIs	https://doi.org/10.1186/1471-2172-11-44
Publication status	Published - 1 Sept 2010

Keywords

Actin Depolymerizing Factors
Actins
Animals
Cell Line
Cloning, Molecular
Cytoskeleton
Enzyme Activation
Humans
Lim Kinases
Mice
Microglia
N-Formylmethionine Leucyl-Phenylalanine
NADPH Oxidase
Polymerization
RNA, Small Interfering
Transgenes

Access to Document

10.1186/1471-2172-11-44

Cite this

@article{04258d19243c44599b3f0679524c5f2a,

title = "Effects of F/G-actin ratio and actin turn-over rate on NADPH oxidase activity in microglia",

abstract = "Most in vivo studies that have addressed the role of actin dynamics in NADPH oxidase function in phagocytes have used toxins to modulate the polymerization state of actin and mostly effects on actin has been evaluated by end point measurements of filamentous actin, which says little about actin dynamics, and without consideration for the subcellular distribution of the perturbed actin cytoskeleton.",

keywords = "Actin Depolymerizing Factors, Actins, Animals, Cell Line, Cloning, Molecular, Cytoskeleton, Enzyme Activation, Humans, Lim Kinases, Mice, Microglia, N-Formylmethionine Leucyl-Phenylalanine, NADPH Oxidase, Polymerization, RNA, Small Interfering, Transgenes",

author = "Izabela Rasmussen and Pedersen, {Line Hjortsh{\o}j} and Luise Byg and Kazuhiro Suzuki and Hideki Sumimoto and Frederik Vilhardt",

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doi = "10.1186/1471-2172-11-44",

language = "English",

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journal = "B M C Immunology",

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TY - JOUR

T1 - Effects of F/G-actin ratio and actin turn-over rate on NADPH oxidase activity in microglia

AU - Rasmussen, Izabela

AU - Pedersen, Line Hjortshøj

AU - Byg, Luise

AU - Suzuki, Kazuhiro

AU - Sumimoto, Hideki

AU - Vilhardt, Frederik

PY - 2010/9/1

Y1 - 2010/9/1

N2 - Most in vivo studies that have addressed the role of actin dynamics in NADPH oxidase function in phagocytes have used toxins to modulate the polymerization state of actin and mostly effects on actin has been evaluated by end point measurements of filamentous actin, which says little about actin dynamics, and without consideration for the subcellular distribution of the perturbed actin cytoskeleton.

AB - Most in vivo studies that have addressed the role of actin dynamics in NADPH oxidase function in phagocytes have used toxins to modulate the polymerization state of actin and mostly effects on actin has been evaluated by end point measurements of filamentous actin, which says little about actin dynamics, and without consideration for the subcellular distribution of the perturbed actin cytoskeleton.

KW - Actin Depolymerizing Factors

KW - Actins

KW - Animals

KW - Cell Line

KW - Cloning, Molecular

KW - Cytoskeleton

KW - Enzyme Activation

KW - Humans

KW - Lim Kinases

KW - Mice

KW - Microglia

KW - N-Formylmethionine Leucyl-Phenylalanine

KW - NADPH Oxidase

KW - Polymerization

KW - RNA, Small Interfering

KW - Transgenes

U2 - 10.1186/1471-2172-11-44

DO - 10.1186/1471-2172-11-44

M3 - Journal article

C2 - 20825680

SN - 1471-2172

VL - 11

SP - 44

JO - B M C Immunology

JF - B M C Immunology

ER -