TY - JOUR
T1 - Efficient discrimination and removal of phospholipids during electromembrane extraction from human plasma samples
AU - Vårdal, Linda
AU - Gjelstad, Astrid
AU - Huang, Chuixiu
AU - Øiestad, Elisabeth Leere
AU - Pedersen-Bjergaard, Stig
PY - 2017
Y1 - 2017
N2 - AIM:
For the first time, extracts obtained from human plasma samples by electromembrane extraction (EME) were investigated comprehensively with particular respect to phospholipids using ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Thhe purpose was to investigate the potential of EME for phospholipid cleanup in different EME systems.
RESULTS & DISCUSSION:
No traces of phospholipids were detected in any of the acceptor solutions, whereas the model analytes were extracted with recoveries up to 50%. Thus, the EME systems tested in this work were found to be highly efficient for providing phospholipid-free extracts.
CONCLUSION:
Ultra-HPLC-MS/MS analysis of the donor solutions revealed that the phospholipids principally remained in the plasma samples. This proved that the phospholipids did not migrate in the electrical field and they were prevented from penetrating the supported liquid membrane.
AB - AIM:
For the first time, extracts obtained from human plasma samples by electromembrane extraction (EME) were investigated comprehensively with particular respect to phospholipids using ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Thhe purpose was to investigate the potential of EME for phospholipid cleanup in different EME systems.
RESULTS & DISCUSSION:
No traces of phospholipids were detected in any of the acceptor solutions, whereas the model analytes were extracted with recoveries up to 50%. Thus, the EME systems tested in this work were found to be highly efficient for providing phospholipid-free extracts.
CONCLUSION:
Ultra-HPLC-MS/MS analysis of the donor solutions revealed that the phospholipids principally remained in the plasma samples. This proved that the phospholipids did not migrate in the electrical field and they were prevented from penetrating the supported liquid membrane.
U2 - 10.4155/bio-2017-0027
DO - 10.4155/bio-2017-0027
M3 - Journal article
C2 - 28508669
SN - 1757-6180
VL - 9
SP - 631
EP - 641
JO - Bioanalysis
JF - Bioanalysis
IS - 8
ER -