Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements

Alicia Lundby; Hiroki Mutoh; Dimitar Dimitrov; Walther Akemann; Thomas Knöpfel

doi:10.1371/journal.pone.0002514

Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements

Alicia Lundby
, Hiroki Mutoh
, Dimitar Dimitrov
, Walther Akemann
, Thomas Knöpfel

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.

Original language	English
Journal	PLoS ONE
Volume	3
Issue number	6
Pages (from-to)	e2514
ISSN	1932-6203
DOIs	https://doi.org/10.1371/journal.pone.0002514
Publication status	Published - 2008

Bibliographical note

Keywords: Animals; Fluorescence; Genetic Engineering; Ion Channel Gating; PC12 Cells; Potassium Channels; Protein Conformation; Rats

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abstract = "Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.",

author = "Alicia Lundby and Hiroki Mutoh and Dimitar Dimitrov and Walther Akemann and Thomas Kn{\"o}pfel",

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AU - Lundby, Alicia

AU - Mutoh, Hiroki

AU - Dimitrov, Dimitar

AU - Akemann, Walther

AU - Knöpfel, Thomas

N1 - Keywords: Animals; Fluorescence; Genetic Engineering; Ion Channel Gating; PC12 Cells; Potassium Channels; Protein Conformation; Rats

PY - 2008

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N2 - Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.

AB - Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.

U2 - 10.1371/journal.pone.0002514

DO - 10.1371/journal.pone.0002514

M3 - Journal article

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