Enzymatic degradation of polymer covered SOPC-liposomes in relation to drug delivery

J. Davidsen; C. Vermehren; S. Frøkjær; O. G. Mouritsen; K. Jørgensen

doi:10.1016/S0001-8686(00)00058-0

Enzymatic degradation of polymer covered SOPC-liposomes in relation to drug delivery

J. Davidsen^*, C. Vermehren, S. Frøkjær, O. G. Mouritsen, K. Jørgensen

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

12 Citations (Scopus)

Abstract

Polyethylenoxide (PEG) covered liposomes are used as lipid-based drug-delivery systems. In comparison to conventional liposomes the polymer-covered liposomes display a long circulation half-life in the blood stream. We investigate the influence of polyethyleneoxide-distearoylphosphatidylethanolamine (DSPE-PEG₇₅₀) lipopolymer concentration on phospholipase A₂ (PLA₂) catalyzed hydrolysis of liposomes composed of stearoyloleoylphosphatidylcholine (SOPC). The characteristic PLA₂ lag-time was determined by fluorescence and the degree of lipid hydrolysis was followed by HPLC analysis. Particle size and zeta-potential were measured as a function of DSPE-PEG₇₅₀ lipopolymer concentration. A significant decrease in the lag-time, and hence an increase in enzyme activity, was observed with increasing concentrations of the anionic DSPE-PEG₇₅₀ lipopolymer lipids. The observed decrease in lag-time might be related to changes in the surface potential and the PLA₂ lipid membrane affinity.

Original language	English
Journal	Advances in Colloid and Interface Science
Volume	89-90
Pages (from-to)	303-311
Number of pages	9
ISSN	0001-8686
DOIs	https://doi.org/10.1016/S0001-8686(00)00058-0
Publication status	Published - 29 Jan 2001

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title = "Enzymatic degradation of polymer covered SOPC-liposomes in relation to drug delivery",

abstract = "Polyethylenoxide (PEG) covered liposomes are used as lipid-based drug-delivery systems. In comparison to conventional liposomes the polymer-covered liposomes display a long circulation half-life in the blood stream. We investigate the influence of polyethyleneoxide-distearoylphosphatidylethanolamine (DSPE-PEG750) lipopolymer concentration on phospholipase A2 (PLA2) catalyzed hydrolysis of liposomes composed of stearoyloleoylphosphatidylcholine (SOPC). The characteristic PLA2 lag-time was determined by fluorescence and the degree of lipid hydrolysis was followed by HPLC analysis. Particle size and zeta-potential were measured as a function of DSPE-PEG750 lipopolymer concentration. A significant decrease in the lag-time, and hence an increase in enzyme activity, was observed with increasing concentrations of the anionic DSPE-PEG750 lipopolymer lipids. The observed decrease in lag-time might be related to changes in the surface potential and the PLA2 lipid membrane affinity.",

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T1 - Enzymatic degradation of polymer covered SOPC-liposomes in relation to drug delivery

AU - Davidsen, J.

AU - Vermehren, C.

AU - Frøkjær, S.

AU - Mouritsen, O. G.

AU - Jørgensen, K.

PY - 2001/1/29

Y1 - 2001/1/29

N2 - Polyethylenoxide (PEG) covered liposomes are used as lipid-based drug-delivery systems. In comparison to conventional liposomes the polymer-covered liposomes display a long circulation half-life in the blood stream. We investigate the influence of polyethyleneoxide-distearoylphosphatidylethanolamine (DSPE-PEG750) lipopolymer concentration on phospholipase A2 (PLA2) catalyzed hydrolysis of liposomes composed of stearoyloleoylphosphatidylcholine (SOPC). The characteristic PLA2 lag-time was determined by fluorescence and the degree of lipid hydrolysis was followed by HPLC analysis. Particle size and zeta-potential were measured as a function of DSPE-PEG750 lipopolymer concentration. A significant decrease in the lag-time, and hence an increase in enzyme activity, was observed with increasing concentrations of the anionic DSPE-PEG750 lipopolymer lipids. The observed decrease in lag-time might be related to changes in the surface potential and the PLA2 lipid membrane affinity.

AB - Polyethylenoxide (PEG) covered liposomes are used as lipid-based drug-delivery systems. In comparison to conventional liposomes the polymer-covered liposomes display a long circulation half-life in the blood stream. We investigate the influence of polyethyleneoxide-distearoylphosphatidylethanolamine (DSPE-PEG750) lipopolymer concentration on phospholipase A2 (PLA2) catalyzed hydrolysis of liposomes composed of stearoyloleoylphosphatidylcholine (SOPC). The characteristic PLA2 lag-time was determined by fluorescence and the degree of lipid hydrolysis was followed by HPLC analysis. Particle size and zeta-potential were measured as a function of DSPE-PEG750 lipopolymer concentration. A significant decrease in the lag-time, and hence an increase in enzyme activity, was observed with increasing concentrations of the anionic DSPE-PEG750 lipopolymer lipids. The observed decrease in lag-time might be related to changes in the surface potential and the PLA2 lipid membrane affinity.

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U2 - 10.1016/S0001-8686(00)00058-0

DO - 10.1016/S0001-8686(00)00058-0

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C2 - 11215800

AN - SCOPUS:0034746681

SN - 0001-8686

VL - 89-90

SP - 303

EP - 311

JO - Advances in Colloid and Interface Science

JF - Advances in Colloid and Interface Science

ER -

Enzymatic degradation of polymer covered SOPC-liposomes in relation to drug delivery

Abstract

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