Enzyme-linked immunosorbent assay for activation of the classical complement pathway by Plasmodium falciparum-infected erythrocyte surface antigen-specific antibodies

Mads Delbo Larsen; Rafael Bayarri-Olmos; Peter Garred; Lars Hviid

doi:10.1007/978-1-0716-2189-9_50

Enzyme-linked immunosorbent assay for activation of the classical complement pathway by Plasmodium falciparum-infected erythrocyte surface antigen-specific antibodies

Mads Delbo Larsen, Rafael Bayarri-Olmos, Peter Garred, Lars Hviid

Research output: Contribution to journal › Journal article › Research › peer-review

1 Citation (Scopus)

Abstract

Enzyme-linked immunosorbent assays (ELISA) have a wide range of applications, ranging from specific antibody titer determination to quantification of any biological or non-biological substance with a specific binding partner (usually an antibody). The activity of biological cascades, such as the complement cascade of the innate immune system, can also be assessed by ELISA. We present here an assay optimized for the detection of the activation of the classical complement pathway by polyclonal and monoclonal antibodies (mAbs) specific for Plasmodium falciparum-infected erythrocyte surface antigens.

Original language	English
Journal	Methods in Molecular Biology
Volume	2470
Pages (from-to)	673-678
Number of pages	6
ISSN	1064-3745
DOIs	https://doi.org/10.1007/978-1-0716-2189-9_50
Publication status	Published - 2022

Bibliographical note

Keywords

Antibodies, Protozoan
Antigens, Protozoan
Antigens, Surface/metabolism
Complement Pathway, Classical
Enzyme-Linked Immunosorbent Assay
Erythrocytes/metabolism
Humans
Malaria, Falciparum
Plasmodium falciparum/metabolism
Protozoan Proteins/metabolism

Access to Document

10.1007/978-1-0716-2189-9_50

Cite this

Enzyme-linked immunosorbent assay for activation of the classical complement pathway by Plasmodium falciparum-infected erythrocyte surface antigen-specific antibodies. / Larsen, Mads Delbo; Bayarri-Olmos, Rafael; Garred, Peter ; Hviid, Lars.

In: Methods in Molecular Biology, Vol. 2470, 2022, p. 673-678.

Research output: Contribution to journal › Journal article › Research › peer-review

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AU - Garred, Peter

AU - Hviid, Lars

PY - 2022

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KW - Malaria, Falciparum

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