TY - JOUR
T1 - Expanding the landscape of diterpene structural diversity by stereochemically controlled combinatorial biosynthesis
AU - Andersen-Ranberg, Johan
AU - Kongstad, Kenneth Thermann
AU - Nielsen, Morten Thrane
AU - Bjerg Jensen, Niels
AU - Pateraki, Irini
AU - Bach, Søren Spanner
AU - Hamberger, Britta
AU - Zerbe, Philipp
AU - Stærk, Dan
AU - Bohlmann, Jörg
AU - Møller, Birger Lindberg
AU - Hamberger, Björn Robert
PY - 2016
Y1 - 2016
N2 - Plant derived diterpenoids are relevant as pharmaceuticals, food additives and fragrances, yet a broader industrial utilization of these bioproducts is limited due to their low natural abundance and high structural complexity. Mimicking the modularity of diterpene biosynthesis in plants, we constructed 51 functional diterpene synthase combinations, 41 of which were “new-to-nature”. Here, we demonstrate stereoselective biosynthesis in Nicotiana benthamiana of 47 diterpene skeletons including natural variants and novel enantiomeric or diastereomeric counterparts. Scalable biotechnological production for four selected industrially relevant targets was realized in engineered strains of Saccharomyces cerevisiae
AB - Plant derived diterpenoids are relevant as pharmaceuticals, food additives and fragrances, yet a broader industrial utilization of these bioproducts is limited due to their low natural abundance and high structural complexity. Mimicking the modularity of diterpene biosynthesis in plants, we constructed 51 functional diterpene synthase combinations, 41 of which were “new-to-nature”. Here, we demonstrate stereoselective biosynthesis in Nicotiana benthamiana of 47 diterpene skeletons including natural variants and novel enantiomeric or diastereomeric counterparts. Scalable biotechnological production for four selected industrially relevant targets was realized in engineered strains of Saccharomyces cerevisiae
U2 - 10.1002/anie.201510650
DO - 10.1002/anie.201510650
M3 - Journal article
C2 - 26749264
VL - 55
SP - 2142
EP - 2146
JO - Angewandte Chemie International Edition
JF - Angewandte Chemie International Edition
SN - 1433-7851
IS - 6
ER -