Flavobacterium sp. strain 4221 and Pedobacter sp. strain 4236 beta-1,3-glucanases that are active at low temperatures

Mikkel A. Rasmussen, Søren Madsen, Peter Stougaard, Mads Grønvald Johnsen

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    Abstract

    Secretion of beta-1,3-glucanases by the arctic bacterial isolates 4221 and 4236, related to the genera Flavobacterium and Pedobacter, was discovered. Escherichia coli and Lactococcus lactis expression of beta-1,3-glucanases Glc4221-1 and Glc4236-1 from the respective isolates was achieved. The enzymes hydrolyzed fungal cell walls and retained activity at low temperatures.
    Original languageEnglish
    JournalApplied and Environmental Microbiology
    Volume74
    Issue number22
    Pages (from-to)7070-7072
    Number of pages3
    ISSN0099-2240
    DOIs
    Publication statusPublished - 2008

    Keywords

    • Amino Acid Sequence
    • Bacteroidetes
    • Base Sequence
    • Cell Wall
    • Cloning, Molecular
    • Cold Temperature
    • Escherichia coli
    • Flavobacterium
    • Gene Expression
    • Glucan 1,3-beta-Glucosidase
    • Lactococcus lactis
    • Molecular Sequence Data
    • Temperature

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