Flow cytometry detection of surface and intracellular antigens in pancreas from a single mouse embryo

Pia Nyeng; Gelo Victoriano Dela Cruz; Tor Henrik Semb

doi:10.1016/j.xpro.2021.100636

Flow cytometry detection of surface and intracellular antigens in pancreas from a single mouse embryo

Pia Nyeng^*, Gelo Victoriano Dela Cruz, Tor Henrik Semb

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

3 Citations (Scopus)

18 Downloads (Pure)

Abstract

We here report a flow-cytometry-based protocol to measure single-cell protein expression in small samples. The protocol is optimized for simultaneous detection of fluorescent proteins and intracellular and surface antigens in the embryonic pancreas from the mouse. Owing to low cell numbers, current protocols for flow cytometric analysis of embryonic tissues rely on tissue pooling. Our protocol enables analysis of one pancreas per sample, thereby facilitating detection of biological variation and minimizing the number of experimental animals needed. For complete details on the use and execution of this protocol, please refer to Nyeng et al (2019).

Original language	English
Article number	100636
Journal	STAR Protocols
Volume	2
Issue number	3
Number of pages	21
ISSN	2666-1667
DOIs	https://doi.org/10.1016/j.xpro.2021.100636
Publication status	Published - 2021

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10.1016/j.xpro.2021.100636Licence: CC BY-NC-ND

Flow cytometry detection of surface and intracellular antigens in pancreas from a single mouse embryoFinal published version, 5.59 MBLicence: CC BY-NC-ND

Cite this

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title = "Flow cytometry detection of surface and intracellular antigens in pancreas from a single mouse embryo",

abstract = "We here report a flow-cytometry-based protocol to measure single-cell protein expression in small samples. The protocol is optimized for simultaneous detection of fluorescent proteins and intracellular and surface antigens in the embryonic pancreas from the mouse. Owing to low cell numbers, current protocols for flow cytometric analysis of embryonic tissues rely on tissue pooling. Our protocol enables analysis of one pancreas per sample, thereby facilitating detection of biological variation and minimizing the number of experimental animals needed. For complete details on the use and execution of this protocol, please refer to Nyeng et al (2019).",

author = "Pia Nyeng and {Dela Cruz}, {Gelo Victoriano} and Semb, {Tor Henrik}",

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T1 - Flow cytometry detection of surface and intracellular antigens in pancreas from a single mouse embryo

AU - Nyeng, Pia

AU - Dela Cruz, Gelo Victoriano

AU - Semb, Tor Henrik

PY - 2021

Y1 - 2021

N2 - We here report a flow-cytometry-based protocol to measure single-cell protein expression in small samples. The protocol is optimized for simultaneous detection of fluorescent proteins and intracellular and surface antigens in the embryonic pancreas from the mouse. Owing to low cell numbers, current protocols for flow cytometric analysis of embryonic tissues rely on tissue pooling. Our protocol enables analysis of one pancreas per sample, thereby facilitating detection of biological variation and minimizing the number of experimental animals needed. For complete details on the use and execution of this protocol, please refer to Nyeng et al (2019).

AB - We here report a flow-cytometry-based protocol to measure single-cell protein expression in small samples. The protocol is optimized for simultaneous detection of fluorescent proteins and intracellular and surface antigens in the embryonic pancreas from the mouse. Owing to low cell numbers, current protocols for flow cytometric analysis of embryonic tissues rely on tissue pooling. Our protocol enables analysis of one pancreas per sample, thereby facilitating detection of biological variation and minimizing the number of experimental animals needed. For complete details on the use and execution of this protocol, please refer to Nyeng et al (2019).

U2 - 10.1016/j.xpro.2021.100636

DO - 10.1016/j.xpro.2021.100636

M3 - Journal article

C2 - 34258596

VL - 2

JO - STAR Protocols

JF - STAR Protocols

SN - 2666-1667

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