Fluispotter, a novel automated and wearable device for accurate volume serial dried blood spot sampling

Khem Bahadur Adhikari; Morten Rohde; Sten Velschow; Ulla Feldt-Rasmussen; Jesper Johannesen; Anders Holten Johnsen

doi:10.4155/bio-2020-0048

Fluispotter, a novel automated and wearable device for accurate volume serial dried blood spot sampling

Khem Bahadur Adhikari, Morten Rohde, Sten Velschow, Ulla Feldt-Rasmussen, Jesper Johannesen, Anders Holten Johnsen

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Aim: A novel automated serial dried blood spot (DBS) sampler, 'Fluispotter', was tested for its sampling performance. Materials & methods: An LC-MS/MS method was developed for the analysis of cortisol in DBS samples serially spotted by Fluispotter. The cortisol concentrations in 148 paired DBS and plasma samples were compared across a hematocrit (HCT) range of 22-55%. Results: The interassay accuracy and precision were <10%. Overall assay bias was negligible across the HCTs tested when analyzing the whole-spot DBS samples. The accuracy and precision of the blood volume in 10 μl DBS samples spotted by Fluispotters and micropipettes were within 3%. Deming regression and Bland-Altman analysis showed a good agreement of DBS-predicted and measured plasma cortisol. Conclusion: The Fluispotter performed serial sampling with high accuracy and precision of the sample blood volume.

Original language	English
Journal	Bioanalysis
Volume	12
Issue number	10
Pages (from-to)	665-681
Number of pages	17
ISSN	1757-6180
DOIs	https://doi.org/10.4155/bio-2020-0048
Publication status	Published - May 2020

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Fluispotter, a novel automated and wearable device for accurate volume serial dried blood spot sampling. / Adhikari, Khem Bahadur; Rohde, Morten; Velschow, Sten; Feldt-Rasmussen, Ulla ; Johannesen, Jesper; Johnsen, Anders Holten.

In: Bioanalysis, Vol. 12, No. 10, 05.2020, p. 665-681.

Research output: Contribution to journal › Journal article › Research › peer-review

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abstract = "Aim: A novel automated serial dried blood spot (DBS) sampler, 'Fluispotter', was tested for its sampling performance. Materials & methods: An LC-MS/MS method was developed for the analysis of cortisol in DBS samples serially spotted by Fluispotter. The cortisol concentrations in 148 paired DBS and plasma samples were compared across a hematocrit (HCT) range of 22-55%. Results: The interassay accuracy and precision were <10%. Overall assay bias was negligible across the HCTs tested when analyzing the whole-spot DBS samples. The accuracy and precision of the blood volume in 10 μl DBS samples spotted by Fluispotters and micropipettes were within 3%. Deming regression and Bland-Altman analysis showed a good agreement of DBS-predicted and measured plasma cortisol. Conclusion: The Fluispotter performed serial sampling with high accuracy and precision of the sample blood volume.",

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AU - Johannesen, Jesper

AU - Johnsen, Anders Holten

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