Functionalized graphene-oxide grids enable high-resolution cryo-EM structures of the SNF2h-nucleosome complex without crosslinking

Un Seng Chio; Eugene Palovcak; Anton A.A. Smith; Henriette Autzen; Elise N. Muñoz; Zanlin Yu; Feng Wang; David A. Agard; Jean Paul Armache; Geeta J. Narlikar; Yifan Cheng

doi:10.1038/s41467-024-46178-y

Functionalized graphene-oxide grids enable high-resolution cryo-EM structures of the SNF2h-nucleosome complex without crosslinking

Un Seng Chio, Eugene Palovcak, Anton A.A. Smith, Henriette Autzen, Elise N. Muñoz, Zanlin Yu, Feng Wang, David A. Agard, Jean Paul Armache, Geeta J. Narlikar^*, Yifan Cheng

^*Corresponding author for this work

Biomolecular Sciences

Research output: Contribution to journal › Journal article › Research › peer-review

1 Citation (Scopus)

13 Downloads (Pure)

Abstract

Nucleosome-protein complexes stick to the air-water interface and denature upon plunge freezing for cryoEM. Here, authors Chio and Palovcak et al. develop EM grids that protect such complexes and use these grids to study the ATP-dependent chromatin remodeler SNF2h.

Original language	English
Article number	2225
Journal	Nature Communications
Volume	15
Issue number	1
Number of pages	12
ISSN	2041-1723
DOIs	https://doi.org/10.1038/s41467-024-46178-y
Publication status	Published - 2024

Bibliographical note

Publisher Copyright:
© The Author(s) 2024.

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Cite this

Functionalized graphene-oxide grids enable high-resolution cryo-EM structures of the SNF2h-nucleosome complex without crosslinking. / Chio, Un Seng; Palovcak, Eugene; Smith, Anton A.A.; Autzen, Henriette; Muñoz, Elise N.; Yu, Zanlin; Wang, Feng; Agard, David A.; Armache, Jean Paul; Narlikar, Geeta J.; Cheng, Yifan.

In: Nature Communications, Vol. 15, No. 1, 2225, 2024.

Research output: Contribution to journal › Journal article › Research › peer-review

Chio, Un Seng ; Palovcak, Eugene ; Smith, Anton A.A. ; Autzen, Henriette ; Muñoz, Elise N. ; Yu, Zanlin ; Wang, Feng ; Agard, David A. ; Armache, Jean Paul ; Narlikar, Geeta J. ; Cheng, Yifan. / Functionalized graphene-oxide grids enable high-resolution cryo-EM structures of the SNF2h-nucleosome complex without crosslinking. In: Nature Communications. 2024 ; Vol. 15, No. 1.

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abstract = "Nucleosome-protein complexes stick to the air-water interface and denature upon plunge freezing for cryoEM. Here, authors Chio and Palovcak et al. develop EM grids that protect such complexes and use these grids to study the ATP-dependent chromatin remodeler SNF2h.",

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AU - Autzen, Henriette

AU - Muñoz, Elise N.

AU - Yu, Zanlin

AU - Wang, Feng

AU - Agard, David A.

AU - Armache, Jean Paul

AU - Narlikar, Geeta J.

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AB - Nucleosome-protein complexes stick to the air-water interface and denature upon plunge freezing for cryoEM. Here, authors Chio and Palovcak et al. develop EM grids that protect such complexes and use these grids to study the ATP-dependent chromatin remodeler SNF2h.

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