Galanin: distribution and effect on contractile activity and release of vasoactive intestinal polypeptide from the isolated perfused porcine ileum

Research output: Contribution to journalJournal articleResearchpeer-review

13 Citations (Scopus)

Abstract

In the pig ileum galanin (GAL)-like immunoreactivity was identified in nerve cell bodies of the submucous plexus and in nerve fibers of the circular and longitudinal muscle layer. Infusion of 5.10(-10)-10(-8) M of GAL into the arterial line of the isolated perfused porcine ileum decreased the frequency of spontaneous phasic contractions in a dose-dependent manner. The frequency of phasic contractions during maximal inhibition by GAL 10(-8) M was 13 +/- 4% (mean +/- SE) of basal frequency (p less than 0.05). The recovery from inhibition by GAL 10(-8) M lasted 16 +/- 1 min. Tonic contractions were not observed in this experimental set-up, neither by standard perfused catheter manometry nor by measurement of cross-sectional area of an intraluminally located balloon. Infusion of GAL 10(-8) M decreased the venous release of vasoactive intestinal polypeptide to 80 +/- 8% of basal release (p less than 0.05). It is concluded that GAL may participate in the regulation of small intestinal motility in the pig.
Original languageEnglish
JournalDigestion
Volume47
Issue number4
Pages (from-to)191-9
Number of pages9
ISSN0012-2823
Publication statusPublished - 1990

Keywords

  • Animals
  • Galanin
  • Gastrointestinal Motility
  • Ileum
  • Neuropeptides
  • Peptides
  • Perfusion
  • Radioimmunoassay
  • Swine
  • Vasoactive Intestinal Peptide

Cite this