Generation of an iPSC-line (BIONi010C-48) with restored P-glycoprotein functionality following transfection with the human MDR1 gene in the AAVS1 locus

Amanda Kongstad Redke Nielsen, Laura Lilieholm-Røngren, Benjamin Schmid, Bjørn Holst, Birger Brodin, Lasse Saaby

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Abstract

The human MDR1 gene encodes the efflux transporter P-glycoprotein, which plays an important part of the blood-brain barrier function of brain microvascular endothelial cells (BMECs). Here, we report the generation of an iPSC line, where a construct of the human MDR1 gene was inserted into the safe-site locus AAVS1. This iPSC line (BIONi010-C-48) shows functional expression of P-gp and can further be differentiated and cultured into electrically tight BMEC-like monolayers exhibiting polarized expression of P-gp in the apical membrane.

Original languageEnglish
Article number103348
JournalStem Cell Research
Volume76
Number of pages5
ISSN1873-5061
DOIs
Publication statusPublished - 2024

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Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.

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