Generation of an iPSC-line (BIONi010C-48) with restored P-glycoprotein functionality following transfection with the human MDR1 gene in the AAVS1 locus

Amanda Kongstad Redke Nielsen; Laura Lilieholm-Røngren; Benjamin Schmid; Bjørn Holst; Birger Brodin; Lasse Saaby

doi:10.1016/j.scr.2024.103348

Generation of an iPSC-line (BIONi010C-48) with restored P-glycoprotein functionality following transfection with the human MDR1 gene in the AAVS1 locus

Amanda Kongstad Redke Nielsen, Laura Lilieholm-Røngren, Benjamin Schmid, Bjørn Holst, Birger Brodin, Lasse Saaby

CNS Drug Delivery and Barrier Modelling

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

The human MDR1 gene encodes the efflux transporter P-glycoprotein, which plays an important part of the blood-brain barrier function of brain microvascular endothelial cells (BMECs). Here, we report the generation of an iPSC line, where a construct of the human MDR1 gene was inserted into the safe-site locus AAVS1. This iPSC line (BIONi010-C-48) shows functional expression of P-gp and can further be differentiated and cultured into electrically tight BMEC-like monolayers exhibiting polarized expression of P-gp in the apical membrane.

Original language	English
Article number	103348
Journal	Stem Cell Research
Volume	76
Number of pages	5
ISSN	1873-5061
DOIs	https://doi.org/10.1016/j.scr.2024.103348
Publication status	Published - 2024

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Generation of an iPSC-line (BIONi010C-48) with restored P-glycoprotein functionality following transfection with the human MDR1 gene in the AAVS1 locus. / Nielsen, Amanda Kongstad Redke; Lilieholm-Røngren, Laura; Schmid, Benjamin; Holst, Bjørn; Brodin, Birger ; Saaby, Lasse.

In: Stem Cell Research, Vol. 76, 103348, 2024.

Research output: Contribution to journal › Journal article › Research › peer-review

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abstract = "The human MDR1 gene encodes the efflux transporter P-glycoprotein, which plays an important part of the blood-brain barrier function of brain microvascular endothelial cells (BMECs). Here, we report the generation of an iPSC line, where a construct of the human MDR1 gene was inserted into the safe-site locus AAVS1. This iPSC line (BIONi010-C-48) shows functional expression of P-gp and can further be differentiated and cultured into electrically tight BMEC-like monolayers exhibiting polarized expression of P-gp in the apical membrane.",

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