TY - JOUR
T1 - Generation of an iPSC-line (BIONi010C-48) with restored P-glycoprotein functionality following transfection with the human MDR1 gene in the AAVS1 locus
AU - Nielsen, Amanda Kongstad Redke
AU - Lilieholm-Røngren, Laura
AU - Schmid, Benjamin
AU - Holst, Bjørn
AU - Brodin, Birger
AU - Saaby, Lasse
N1 - Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
PY - 2024
Y1 - 2024
N2 - The human MDR1 gene encodes the efflux transporter P-glycoprotein, which plays an important part of the blood-brain barrier function of brain microvascular endothelial cells (BMECs). Here, we report the generation of an iPSC line, where a construct of the human MDR1 gene was inserted into the safe-site locus AAVS1. This iPSC line (BIONi010-C-48) shows functional expression of P-gp and can further be differentiated and cultured into electrically tight BMEC-like monolayers exhibiting polarized expression of P-gp in the apical membrane.
AB - The human MDR1 gene encodes the efflux transporter P-glycoprotein, which plays an important part of the blood-brain barrier function of brain microvascular endothelial cells (BMECs). Here, we report the generation of an iPSC line, where a construct of the human MDR1 gene was inserted into the safe-site locus AAVS1. This iPSC line (BIONi010-C-48) shows functional expression of P-gp and can further be differentiated and cultured into electrically tight BMEC-like monolayers exhibiting polarized expression of P-gp in the apical membrane.
U2 - 10.1016/j.scr.2024.103348
DO - 10.1016/j.scr.2024.103348
M3 - Journal article
C2 - 38364505
VL - 76
JO - Stem Cell Research
JF - Stem Cell Research
SN - 1873-5061
M1 - 103348
ER -