Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson's disease patients carrying the heterozygous mutations c.815G > A (p.R272Q) or c.1348C > T (p.R450C) in the RHOT1 gene encoding Miro1

Axel Chemla; Giuseppe Arena; Gizem Onal; Jonas Walter; Clara Berenguer-Escuder; Dajana Grossmann; Anne Grünewald; Jens C. Schwamborn; Rejko Krüger

doi:10.1016/j.scr.2023.103145

Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson's disease patients carrying the heterozygous mutations c.815G > A (p.R272Q) or c.1348C > T (p.R450C) in the RHOT1 gene encoding Miro1

Axel Chemla, Giuseppe Arena^*, Gizem Onal, Jonas Walter, Clara Berenguer-Escuder, Dajana Grossmann, Anne Grünewald, Jens C. Schwamborn, Rejko Krüger

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Fibroblasts from two Parkinson's disease (PD) patients carrying either the heterozygous mutation c.815G > A (Miro1 p.R272Q) or c.1348C > T (Miro1 p.R450C) in the RHOT1 gene, were converted into induced pluripotent stem cells (iPSCs) using RNA-based and episomal reprogramming, respectively. The corresponding isogenic gene-corrected lines have been generated using CRISPR/Cas9 technology. These two isogenic pairs will be used to study Miro1-related molecular mechanisms underlying neurodegeneration in relevant iPSC-derived neuronal models (e.g., midbrain dopaminergic neurons and astrocytes).

Original language	English
Article number	103145
Journal	Stem Cell Research
Volume	71
Number of pages	6
ISSN	1873-5061
DOIs	https://doi.org/10.1016/j.scr.2023.103145
Publication status	Published - 2023
Externally published	Yes

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Publisher Copyright:
© 2023 The Author(s)

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Chemla, A., Arena, G., Onal, G., Walter, J., Berenguer-Escuder, C., Grossmann, D., Grünewald, A., Schwamborn, J. C., & Krüger, R. (2023). Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson's disease patients carrying the heterozygous mutations c.815G > A (p.R272Q) or c.1348C > T (p.R450C) in the RHOT1 gene encoding Miro1. Stem Cell Research, 71, [103145]. https://doi.org/10.1016/j.scr.2023.103145

Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson's disease patients carrying the heterozygous mutations c.815G > A (p.R272Q) or c.1348C > T (p.R450C) in the RHOT1 gene encoding Miro1. / Chemla, Axel; Arena, Giuseppe; Onal, Gizem; Walter, Jonas; Berenguer-Escuder, Clara; Grossmann, Dajana; Grünewald, Anne; Schwamborn, Jens C.; Krüger, Rejko.

In: Stem Cell Research, Vol. 71, 103145, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Chemla, A, Arena, G, Onal, G, Walter, J, Berenguer-Escuder, C, Grossmann, D, Grünewald, A, Schwamborn, JC & Krüger, R 2023, 'Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson's disease patients carrying the heterozygous mutations c.815G > A (p.R272Q) or c.1348C > T (p.R450C) in the RHOT1 gene encoding Miro1', Stem Cell Research, vol. 71, 103145. https://doi.org/10.1016/j.scr.2023.103145

Chemla A, Arena G, Onal G, Walter J, Berenguer-Escuder C, Grossmann D et al. Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson's disease patients carrying the heterozygous mutations c.815G > A (p.R272Q) or c.1348C > T (p.R450C) in the RHOT1 gene encoding Miro1. Stem Cell Research. 2023;71. 103145. https://doi.org/10.1016/j.scr.2023.103145

Chemla, Axel ; Arena, Giuseppe ; Onal, Gizem ; Walter, Jonas ; Berenguer-Escuder, Clara ; Grossmann, Dajana ; Grünewald, Anne ; Schwamborn, Jens C. ; Krüger, Rejko. / Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson's disease patients carrying the heterozygous mutations c.815G > A (p.R272Q) or c.1348C > T (p.R450C) in the RHOT1 gene encoding Miro1. In: Stem Cell Research. 2023 ; Vol. 71.

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abstract = "Fibroblasts from two Parkinson's disease (PD) patients carrying either the heterozygous mutation c.815G > A (Miro1 p.R272Q) or c.1348C > T (Miro1 p.R450C) in the RHOT1 gene, were converted into induced pluripotent stem cells (iPSCs) using RNA-based and episomal reprogramming, respectively. The corresponding isogenic gene-corrected lines have been generated using CRISPR/Cas9 technology. These two isogenic pairs will be used to study Miro1-related molecular mechanisms underlying neurodegeneration in relevant iPSC-derived neuronal models (e.g., midbrain dopaminergic neurons and astrocytes).",

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AU - Chemla, Axel

AU - Arena, Giuseppe

AU - Onal, Gizem

AU - Walter, Jonas

AU - Berenguer-Escuder, Clara

AU - Grossmann, Dajana

AU - Grünewald, Anne

AU - Schwamborn, Jens C.

AU - Krüger, Rejko

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AB - Fibroblasts from two Parkinson's disease (PD) patients carrying either the heterozygous mutation c.815G > A (Miro1 p.R272Q) or c.1348C > T (Miro1 p.R450C) in the RHOT1 gene, were converted into induced pluripotent stem cells (iPSCs) using RNA-based and episomal reprogramming, respectively. The corresponding isogenic gene-corrected lines have been generated using CRISPR/Cas9 technology. These two isogenic pairs will be used to study Miro1-related molecular mechanisms underlying neurodegeneration in relevant iPSC-derived neuronal models (e.g., midbrain dopaminergic neurons and astrocytes).

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DO - 10.1016/j.scr.2023.103145

M3 - Journal article

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JO - Stem Cell Research

JF - Stem Cell Research

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