TY - JOUR
T1 - Generic GPCR residue numbers - aligning topology maps while minding the gaps
AU - Isberg, Vignir
AU - de Graaf, Chris
AU - Bortolato, Andrea
AU - Cherezov, Vadim
AU - Katritch, Vsevolod
AU - Marshall, Fiona H
AU - Mordalski, Stefan
AU - Pin, Jean-Philippe
AU - Stevens, Raymond C
AU - Vriend, Gerrit
AU - Gloriam, David E
N1 - Copyright © 2014 Elsevier Ltd. All rights reserved.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Generic residue numbers facilitate comparisons of, for example, mutational effects, ligand interactions, and structural motifs. The numbering scheme by Ballesteros and Weinstein for residues within the class A GPCRs (G protein-coupled receptors) has more than 1100 citations, and the recent crystal structures for classes B, C, and F now call for a community consensus in residue numbering within and across these classes. Furthermore, the structural era has uncovered helix bulges and constrictions that offset the generic residue numbers. The use of generic residue numbers depends on convenient access by pharmacologists, chemists, and structural biologists. We review the generic residue numbering schemes for each GPCR class, as well as a complementary structure-based scheme, and provide illustrative examples and GPCR database (GPCRDB) web tools to number any receptor sequence or structure.
AB - Generic residue numbers facilitate comparisons of, for example, mutational effects, ligand interactions, and structural motifs. The numbering scheme by Ballesteros and Weinstein for residues within the class A GPCRs (G protein-coupled receptors) has more than 1100 citations, and the recent crystal structures for classes B, C, and F now call for a community consensus in residue numbering within and across these classes. Furthermore, the structural era has uncovered helix bulges and constrictions that offset the generic residue numbers. The use of generic residue numbers depends on convenient access by pharmacologists, chemists, and structural biologists. We review the generic residue numbering schemes for each GPCR class, as well as a complementary structure-based scheme, and provide illustrative examples and GPCR database (GPCRDB) web tools to number any receptor sequence or structure.
U2 - 10.1016/j.tips.2014.11.001
DO - 10.1016/j.tips.2014.11.001
M3 - Journal article
C2 - 25541108
VL - 36
SP - 22
EP - 31
JO - Trends in Pharmacological Sciences
JF - Trends in Pharmacological Sciences
SN - 0165-6147
IS - 1
ER -