Generic tools to assess genuine carbohydrate specific effects on in vitro immune modulation exemplified by β-glucans

Anne Rieder; Stine Grimmer; Finn L. Aachmann; Bjørge Westereng; Svein Olav Kolset; Svein Halvor Knutsen

doi:10.1016/j.carbpol.2012.11.092

Generic tools to assess genuine carbohydrate specific effects on in vitro immune modulation exemplified by β-glucans

Anne Rieder, Stine Grimmer, Finn L. Aachmann, Bjørge Westereng, Svein Olav Kolset, Svein Halvor Knutsen

Research output: Contribution to journal › Journal article › Research › peer-review

12 Citations (Scopus)

Abstract

Even if carbohydrate preparations from plant/fungal sources have a high degree of purity, observed immune-stimulation may be caused by minute sample contaminations. Using the example of different β-glucans we present a range of analytical tools crucial for validation of possible immune-stimulatory effects. Two yeast (MacroGard and Zymosan) and one cereal β-glucan (CBG40) increased IL-8 secretion by HT-29 cells considerably. Degradation of the β-glucan samples with β-glucan specific enzymes did hardly influence the effect of Zymosan and CBG40 but significantly decreased the effect of MacroGard. Stimulation of IL-8 secretion by CBG40 and Zymosan was hence not due to their β-glucan content. Instead, the effect of the CBG40 sample was due to low levels of LPS despite the inability of the known LPS inhibitor Polymyxin B to supress its stimulatory effect. We conclude that targeted enzymatic degradation of samples is a powerful validation tool to investigate carbohydrate specific immune-modulation.

Original language	English
Journal	Carbohydrate Polymers
Volume	92
Issue number	2
Pages (from-to)	2075-2083
Number of pages	9
ISSN	0144-8617
DOIs	https://doi.org/10.1016/j.carbpol.2012.11.092
Publication status	Published - 2013

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title = "Generic tools to assess genuine carbohydrate specific effects on in vitro immune modulation exemplified by β-glucans",

abstract = "Even if carbohydrate preparations from plant/fungal sources have a high degree of purity, observed immune-stimulation may be caused by minute sample contaminations. Using the example of different β-glucans we present a range of analytical tools crucial for validation of possible immune-stimulatory effects. Two yeast (MacroGard and Zymosan) and one cereal β-glucan (CBG40) increased IL-8 secretion by HT-29 cells considerably. Degradation of the β-glucan samples with β-glucan specific enzymes did hardly influence the effect of Zymosan and CBG40 but significantly decreased the effect of MacroGard. Stimulation of IL-8 secretion by CBG40 and Zymosan was hence not due to their β-glucan content. Instead, the effect of the CBG40 sample was due to low levels of LPS despite the inability of the known LPS inhibitor Polymyxin B to supress its stimulatory effect. We conclude that targeted enzymatic degradation of samples is a powerful validation tool to investigate carbohydrate specific immune-modulation.",

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doi = "10.1016/j.carbpol.2012.11.092",

language = "English",

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T1 - Generic tools to assess genuine carbohydrate specific effects on in vitro immune modulation exemplified by β-glucans

AU - Rieder, Anne

AU - Grimmer, Stine

AU - Aachmann, Finn L.

AU - Westereng, Bjørge

AU - Kolset, Svein Olav

AU - Knutsen, Svein Halvor

PY - 2013

Y1 - 2013

N2 - Even if carbohydrate preparations from plant/fungal sources have a high degree of purity, observed immune-stimulation may be caused by minute sample contaminations. Using the example of different β-glucans we present a range of analytical tools crucial for validation of possible immune-stimulatory effects. Two yeast (MacroGard and Zymosan) and one cereal β-glucan (CBG40) increased IL-8 secretion by HT-29 cells considerably. Degradation of the β-glucan samples with β-glucan specific enzymes did hardly influence the effect of Zymosan and CBG40 but significantly decreased the effect of MacroGard. Stimulation of IL-8 secretion by CBG40 and Zymosan was hence not due to their β-glucan content. Instead, the effect of the CBG40 sample was due to low levels of LPS despite the inability of the known LPS inhibitor Polymyxin B to supress its stimulatory effect. We conclude that targeted enzymatic degradation of samples is a powerful validation tool to investigate carbohydrate specific immune-modulation.

AB - Even if carbohydrate preparations from plant/fungal sources have a high degree of purity, observed immune-stimulation may be caused by minute sample contaminations. Using the example of different β-glucans we present a range of analytical tools crucial for validation of possible immune-stimulatory effects. Two yeast (MacroGard and Zymosan) and one cereal β-glucan (CBG40) increased IL-8 secretion by HT-29 cells considerably. Degradation of the β-glucan samples with β-glucan specific enzymes did hardly influence the effect of Zymosan and CBG40 but significantly decreased the effect of MacroGard. Stimulation of IL-8 secretion by CBG40 and Zymosan was hence not due to their β-glucan content. Instead, the effect of the CBG40 sample was due to low levels of LPS despite the inability of the known LPS inhibitor Polymyxin B to supress its stimulatory effect. We conclude that targeted enzymatic degradation of samples is a powerful validation tool to investigate carbohydrate specific immune-modulation.

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DO - 10.1016/j.carbpol.2012.11.092

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VL - 92

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JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

IS - 2

ER -

Generic tools to assess genuine carbohydrate specific effects on in vitro immune modulation exemplified by β-glucans

Abstract

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