Genetic analyses in the hyperthermophilic archaeon Sulfolobus islandicus

Qunxin She; Changyi Zhang; Ling Deng; Nan Peng; Zhengjun Chen; Yun Xiang Liang

doi:10.1042/BST0370092

Genetic analyses in the hyperthermophilic archaeon Sulfolobus islandicus

Qunxin She, Changyi Zhang, Ling Deng, Nan Peng, Zhengjun Chen, Yun Xiang Liang

Functional Genomics

Research output: Contribution to journal › Journal article › Research › peer-review

55 Citations (Scopus)

Abstract

Sulfolobus belongs to the hyperthermophilic archaea and it serves as a model organism to study archaeal molecular biology and evolution. In the last few years, we have focused on developing genetic systems for Sulfolobus islandicus using pyrEF as a selection marker and versatile genetic tools have been developed, including methods for constructing gene knockouts and for identifying essential genes. These genetic tools enable us to conduct genetic analysis on the functions of the genes involved in DNA replication and repair processes in S. islandicus and they should also facilitate in vivo analysis of functions of other genes in this model organism.

Original language	English
Journal	Biochemical Society Transactions
Volume	37
Issue number	Pt 1
Pages (from-to)	92-6
Number of pages	4
ISSN	0300-5127
DOIs	https://doi.org/10.1042/BST0370092
Publication status	Published - 2009

Bibliographical note

Keywords: Gene Knockout Techniques; Genes, Reporter; Mutation; Promoter Regions, Genetic; Selection (Genetics); Sulfolobus

Access to Document

10.1042/BST0370092

Cite this

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title = "Genetic analyses in the hyperthermophilic archaeon Sulfolobus islandicus",

abstract = "Sulfolobus belongs to the hyperthermophilic archaea and it serves as a model organism to study archaeal molecular biology and evolution. In the last few years, we have focused on developing genetic systems for Sulfolobus islandicus using pyrEF as a selection marker and versatile genetic tools have been developed, including methods for constructing gene knockouts and for identifying essential genes. These genetic tools enable us to conduct genetic analysis on the functions of the genes involved in DNA replication and repair processes in S. islandicus and they should also facilitate in vivo analysis of functions of other genes in this model organism.",

author = "Qunxin She and Changyi Zhang and Ling Deng and Nan Peng and Zhengjun Chen and Liang, {Yun Xiang}",

note = "Keywords: Gene Knockout Techniques; Genes, Reporter; Mutation; Promoter Regions, Genetic; Selection (Genetics); Sulfolobus",

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T1 - Genetic analyses in the hyperthermophilic archaeon Sulfolobus islandicus

AU - She, Qunxin

AU - Zhang, Changyi

AU - Deng, Ling

AU - Peng, Nan

AU - Chen, Zhengjun

AU - Liang, Yun Xiang

N1 - Keywords: Gene Knockout Techniques; Genes, Reporter; Mutation; Promoter Regions, Genetic; Selection (Genetics); Sulfolobus

PY - 2009

Y1 - 2009

N2 - Sulfolobus belongs to the hyperthermophilic archaea and it serves as a model organism to study archaeal molecular biology and evolution. In the last few years, we have focused on developing genetic systems for Sulfolobus islandicus using pyrEF as a selection marker and versatile genetic tools have been developed, including methods for constructing gene knockouts and for identifying essential genes. These genetic tools enable us to conduct genetic analysis on the functions of the genes involved in DNA replication and repair processes in S. islandicus and they should also facilitate in vivo analysis of functions of other genes in this model organism.

AB - Sulfolobus belongs to the hyperthermophilic archaea and it serves as a model organism to study archaeal molecular biology and evolution. In the last few years, we have focused on developing genetic systems for Sulfolobus islandicus using pyrEF as a selection marker and versatile genetic tools have been developed, including methods for constructing gene knockouts and for identifying essential genes. These genetic tools enable us to conduct genetic analysis on the functions of the genes involved in DNA replication and repair processes in S. islandicus and they should also facilitate in vivo analysis of functions of other genes in this model organism.

U2 - 10.1042/BST0370092

DO - 10.1042/BST0370092

M3 - Journal article

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JO - Biochemical Society Transactions

JF - Biochemical Society Transactions

IS - Pt 1

ER -