Abstract
Novel methods for sampling and characterizing biodiversity hold great promise for re-evaluating patterns of life across the planet. The sampling of airborne spores with a cyclone sampler, and the sequencing of their DNA, have been suggested as an efficient and well-calibrated tool for surveying fungal diversity across various environments. Here we present data originating from the Global Spore Sampling Project, comprising 2,768 samples collected during two years at 47 outdoor locations across the world. Each sample represents fungal DNA extracted from 24 m 3 of air. We applied a conservative bioinformatics pipeline that filtered out sequences that did not show strong evidence of representing a fungal species. The pipeline yielded 27,954 species-level operational taxonomic units (OTUs). Each OTU is accompanied by a probabilistic taxonomic classification, validated through comparison with expert evaluations. To examine the potential of the data for ecological analyses, we partitioned the variation in species distributions into spatial and seasonal components, showing a strong effect of the annual mean temperature on community composition.
| Original language | English |
|---|---|
| Article number | 561 |
| Journal | Scientific Data |
| Volume | 11 |
| Number of pages | 17 |
| ISSN | 2052-4463 |
| DOIs | |
| Publication status | Published - 2024 |
Bibliographical note
© 2024. The Author(s).Keywords
- Air Microbiology
- Spores, Fungal
- DNA, Fungal/analysis
- Fungi/genetics
- Biodiversity
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Global Spore Sampling Project : A global, standardized dataset of airborne fungal DNA. / Ovaskainen, Otso; Abrego, Nerea; Furneaux, Brendan; Hardwick, Bess; Somervuo, Panu; Palorinne, Isabella; Andrew, Nigel R.; Babiy, Ulyana V.; Bao, Tan; Bazzano, Gisela; Bondarchuk, Svetlana N.; Bonebrake, Timothy C.; Brennan, Georgina L.; Bret-Harte, Syndonia; Bässler, Claus; Cagnolo, Luciano; Cameron, Erin K; Chapurlat, Elodie; Creer, Simon; D'Acqui, Luigi P.; de Vere, Natasha; Desprez-Loustau, Marie-Laure; Dongmo, Michel A K; Jacobsen, Ida B. Dyrholm; Fisher, Brian L; de Jesus, Miguel Flores; Gilbert, Gregory S.; Griffith, Gareth W.; Gritsuk, Anna A.; Gross, Andrin; Grudd, Håkan; Halme, Panu; Hanna, Rachid; Hansen, Jannik; Hansen, Lars Holst; Hegbe, Apollon D. M. T.; Hill, Sarah; Hogg, Ian D.; Hultman, Jenni; Hyde, Kevin D.; Hynson, Nicole A.; Ivanova, Natalia; Karisto, Petteri; Kerdraon, Deirdre; Knorre, Anastasia; Krisai-Greilhuber, Irmgard; Kurhinen, Juri; Kuzmina, Masha; Lecomte, Nicolas; Lecomte, Erin; Loaiza, Viviana; Lundin, Erik; Meire, Alexander; Mešić, Armin; Miettinen, Otto; Monkhause, Norman; Mortimer, Peter; Müller, Jörg; Nilsson, R. Henrik; Nonti, Puani Yannick C.; Nordén, Jenni; Nordén, Björn; Paz, Claudia; Pellikka, Petri; Pereira, Danilo; Petch, Geoff; Pitkänen, Juha-Matti; Popa, Flavius; Potter, Caitlin; Purhonen, Jenna; Pätsi, Sanna; Rafiq, Abdullah; Raharinjanahary, Dimby; Rakos, Niklas; Rathnayaka, Achala R.; Raundrup, Katrine; Rebriev, Yury A.; Rikkinen, Jouko; Rogers, Hanna M. K.; Rogovsky, Andrey; Rozhkov, Yuri; Runnel, Kadri; Saarto, Annika; Savchenko, Anton; Schlegel, Markus; Schmidt, Niels Martin; Seibold, Sebastian; Skjøth, Carsten; Stengel, Elisa; Sutyrina, Svetlana V.; Syvänperä, Ilkka; Tedersoo, Leho; Timm, Jebidiah; Tipton, Laura; Toju, Hirokazu; Uscka-Perzanowska, Maria; van der Bank, Michelle; van der Bank, F. Herman; Vandenbrink, Bryan; Ventura, Stefano; Vignisson, Solvi R.; Wang, Xiaoyang; Weisser, Wolfgang W.; Wijesinghe, Subodini N.; Wright, S. Joseph; Yang, Chunyan; Yorou, Nourou S.; Young, Amanda; Yu, Douglas W.; Zakharov, Evgeny V.; Hebert, Paul D. N.; Roslin, Tomas.
In: Scientific Data, Vol. 11, 561, 2024.Research output: Contribution to journal › Journal article › Research › peer-review
}
TY - JOUR
T1 - Global Spore Sampling Project
T2 - A global, standardized dataset of airborne fungal DNA
AU - Ovaskainen, Otso
AU - Abrego, Nerea
AU - Furneaux, Brendan
AU - Hardwick, Bess
AU - Somervuo, Panu
AU - Palorinne, Isabella
AU - Andrew, Nigel R.
AU - Babiy, Ulyana V.
AU - Bao, Tan
AU - Bazzano, Gisela
AU - Bondarchuk, Svetlana N.
AU - Bonebrake, Timothy C.
AU - Brennan, Georgina L.
AU - Bret-Harte, Syndonia
AU - Bässler, Claus
AU - Cagnolo, Luciano
AU - Cameron, Erin K
AU - Chapurlat, Elodie
AU - Creer, Simon
AU - D'Acqui, Luigi P.
AU - de Vere, Natasha
AU - Desprez-Loustau, Marie-Laure
AU - Dongmo, Michel A K
AU - Jacobsen, Ida B. Dyrholm
AU - Fisher, Brian L
AU - de Jesus, Miguel Flores
AU - Gilbert, Gregory S.
AU - Griffith, Gareth W.
AU - Gritsuk, Anna A.
AU - Gross, Andrin
AU - Grudd, Håkan
AU - Halme, Panu
AU - Hanna, Rachid
AU - Hansen, Jannik
AU - Hansen, Lars Holst
AU - Hegbe, Apollon D. M. T.
AU - Hill, Sarah
AU - Hogg, Ian D.
AU - Hultman, Jenni
AU - Hyde, Kevin D.
AU - Hynson, Nicole A.
AU - Ivanova, Natalia
AU - Karisto, Petteri
AU - Kerdraon, Deirdre
AU - Knorre, Anastasia
AU - Krisai-Greilhuber, Irmgard
AU - Kurhinen, Juri
AU - Kuzmina, Masha
AU - Lecomte, Nicolas
AU - Lecomte, Erin
AU - Loaiza, Viviana
AU - Lundin, Erik
AU - Meire, Alexander
AU - Mešić, Armin
AU - Miettinen, Otto
AU - Monkhause, Norman
AU - Mortimer, Peter
AU - Müller, Jörg
AU - Nilsson, R. Henrik
AU - Nonti, Puani Yannick C.
AU - Nordén, Jenni
AU - Nordén, Björn
AU - Paz, Claudia
AU - Pellikka, Petri
AU - Pereira, Danilo
AU - Petch, Geoff
AU - Pitkänen, Juha-Matti
AU - Popa, Flavius
AU - Potter, Caitlin
AU - Purhonen, Jenna
AU - Pätsi, Sanna
AU - Rafiq, Abdullah
AU - Raharinjanahary, Dimby
AU - Rakos, Niklas
AU - Rathnayaka, Achala R.
AU - Raundrup, Katrine
AU - Rebriev, Yury A.
AU - Rikkinen, Jouko
AU - Rogers, Hanna M. K.
AU - Rogovsky, Andrey
AU - Rozhkov, Yuri
AU - Runnel, Kadri
AU - Saarto, Annika
AU - Savchenko, Anton
AU - Schlegel, Markus
AU - Schmidt, Niels Martin
AU - Seibold, Sebastian
AU - Skjøth, Carsten
AU - Stengel, Elisa
AU - Sutyrina, Svetlana V.
AU - Syvänperä, Ilkka
AU - Tedersoo, Leho
AU - Timm, Jebidiah
AU - Tipton, Laura
AU - Toju, Hirokazu
AU - Uscka-Perzanowska, Maria
AU - van der Bank, Michelle
AU - van der Bank, F. Herman
AU - Vandenbrink, Bryan
AU - Ventura, Stefano
AU - Vignisson, Solvi R.
AU - Wang, Xiaoyang
AU - Weisser, Wolfgang W.
AU - Wijesinghe, Subodini N.
AU - Wright, S. Joseph
AU - Yang, Chunyan
AU - Yorou, Nourou S.
AU - Young, Amanda
AU - Yu, Douglas W.
AU - Zakharov, Evgeny V.
AU - Hebert, Paul D. N.
AU - Roslin, Tomas
N1 - © 2024. The Author(s).
PY - 2024
Y1 - 2024
N2 - Novel methods for sampling and characterizing biodiversity hold great promise for re-evaluating patterns of life across the planet. The sampling of airborne spores with a cyclone sampler, and the sequencing of their DNA, have been suggested as an efficient and well-calibrated tool for surveying fungal diversity across various environments. Here we present data originating from the Global Spore Sampling Project, comprising 2,768 samples collected during two years at 47 outdoor locations across the world. Each sample represents fungal DNA extracted from 24 m 3 of air. We applied a conservative bioinformatics pipeline that filtered out sequences that did not show strong evidence of representing a fungal species. The pipeline yielded 27,954 species-level operational taxonomic units (OTUs). Each OTU is accompanied by a probabilistic taxonomic classification, validated through comparison with expert evaluations. To examine the potential of the data for ecological analyses, we partitioned the variation in species distributions into spatial and seasonal components, showing a strong effect of the annual mean temperature on community composition.
AB - Novel methods for sampling and characterizing biodiversity hold great promise for re-evaluating patterns of life across the planet. The sampling of airborne spores with a cyclone sampler, and the sequencing of their DNA, have been suggested as an efficient and well-calibrated tool for surveying fungal diversity across various environments. Here we present data originating from the Global Spore Sampling Project, comprising 2,768 samples collected during two years at 47 outdoor locations across the world. Each sample represents fungal DNA extracted from 24 m 3 of air. We applied a conservative bioinformatics pipeline that filtered out sequences that did not show strong evidence of representing a fungal species. The pipeline yielded 27,954 species-level operational taxonomic units (OTUs). Each OTU is accompanied by a probabilistic taxonomic classification, validated through comparison with expert evaluations. To examine the potential of the data for ecological analyses, we partitioned the variation in species distributions into spatial and seasonal components, showing a strong effect of the annual mean temperature on community composition.
KW - Air Microbiology
KW - Spores, Fungal
KW - DNA, Fungal/analysis
KW - Fungi/genetics
KW - Biodiversity
U2 - 10.1038/s41597-024-03410-0
DO - 10.1038/s41597-024-03410-0
M3 - Journal article
C2 - 38816458
VL - 11
JO - Scientific data
JF - Scientific data
SN - 2052-4463
M1 - 561
ER -