TY - JOUR
T1 - High-intensity interval training remodels the proteome and acetylome of human skeletal muscle
AU - Hostrup, Morten
AU - Lemminger, Anders Krogh
AU - Stocks, Ben
AU - Gonzalez-Franquesa, Alba
AU - Larsen, Jeppe Kjærgaard
AU - Quesada, Julia Prats
AU - Thomassen, Martin
AU - Weinert, Brian Tate
AU - Bangsbo, Jens
AU - Deshmukh, Atul Shahaji
N1 - © 2022, Hostrup et al.
PY - 2022
Y1 - 2022
N2 - Exercise is an effective strategy in the prevention and treatment of metabolic diseases. Alterations in the skeletal muscle proteome, including post-translational modifications, regulate its metabolic adaptations to exercise. Here, we examined the effect of high-intensity interval training (HIIT) on the proteome and acetylome of human skeletal muscle, revealing the response of 3168 proteins and 1263 lysine acetyl-sites on 464 acetylated proteins. We identified global protein adaptations to exercise training involved in metabolism, excitation-contraction coupling, and myofibrillar calcium sensitivity. Furthermore, HIIT increased the acetylation of mitochondrial proteins, particularly those of complex V. We also highlight the regulation of exercise-responsive histone acetyl-sites. These data demonstrate the plasticity of the skeletal muscle proteome and acetylome, providing insight into the regulation of contractile, metabolic and transcriptional processes within skeletal muscle. Herein, we provide a substantial hypothesis-generating resource to stimulate further mechanistic research investigating how exercise improves metabolic health.
AB - Exercise is an effective strategy in the prevention and treatment of metabolic diseases. Alterations in the skeletal muscle proteome, including post-translational modifications, regulate its metabolic adaptations to exercise. Here, we examined the effect of high-intensity interval training (HIIT) on the proteome and acetylome of human skeletal muscle, revealing the response of 3168 proteins and 1263 lysine acetyl-sites on 464 acetylated proteins. We identified global protein adaptations to exercise training involved in metabolism, excitation-contraction coupling, and myofibrillar calcium sensitivity. Furthermore, HIIT increased the acetylation of mitochondrial proteins, particularly those of complex V. We also highlight the regulation of exercise-responsive histone acetyl-sites. These data demonstrate the plasticity of the skeletal muscle proteome and acetylome, providing insight into the regulation of contractile, metabolic and transcriptional processes within skeletal muscle. Herein, we provide a substantial hypothesis-generating resource to stimulate further mechanistic research investigating how exercise improves metabolic health.
U2 - 10.7554/eLife.69802
DO - 10.7554/eLife.69802
M3 - Journal article
C2 - 35638262
VL - 11
JO - eLife
JF - eLife
SN - 2050-084X
M1 - 69802
ER -