High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging

Cristina Isabel Amador; Rune Overlund Stannius; Henriette Lyng Røder; Mette Burmølle

doi:10.1016/j.mimet.2021.106343

High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging

Cristina Isabel Amador, Rune Overlund Stannius, Henriette Lyng Røder, Mette Burmølle

Microbiology

Research output: Contribution to journal › Letter › Research › peer-review

20 Citations (Scopus)

49 Downloads (Pure)

Abstract

The crystal violet assay is widely used for biofilm quantitation despite its toxicity and variability. Here, we instead combine fluorescence labelling with the Cytation 5 multi-mode plate reader, to enable simultaneous acquisition of both quantitative and imaging biofilm data. This high-throughput method produces more robust data and provides information about morphology and spatial species organization within the biofilm.

Original language	English
Article number	106343
Journal	Journal of Microbiological Methods
Volume	190
Number of pages	5
ISSN	0167-7012
DOIs	https://doi.org/10.1016/j.mimet.2021.106343
Publication status	Published - 2021

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10.1016/j.mimet.2021.106343Licence: CC BY

High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imagingFinal published version, 1.01 MBLicence: CC BY

Cite this

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title = "High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging",

abstract = "The crystal violet assay is widely used for biofilm quantitation despite its toxicity and variability. Here, we instead combine fluorescence labelling with the Cytation 5 multi-mode plate reader, to enable simultaneous acquisition of both quantitative and imaging biofilm data. This high-throughput method produces more robust data and provides information about morphology and spatial species organization within the biofilm.",

author = "Amador, {Cristina Isabel} and Stannius, {Rune Overlund} and R{\o}der, {Henriette Lyng} and Mette Burm{\o}lle",

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journal = "Journal of Microbiological Methods",

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T1 - High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging

AU - Amador, Cristina Isabel

AU - Stannius, Rune Overlund

AU - Røder, Henriette Lyng

AU - Burmølle, Mette

PY - 2021

Y1 - 2021

N2 - The crystal violet assay is widely used for biofilm quantitation despite its toxicity and variability. Here, we instead combine fluorescence labelling with the Cytation 5 multi-mode plate reader, to enable simultaneous acquisition of both quantitative and imaging biofilm data. This high-throughput method produces more robust data and provides information about morphology and spatial species organization within the biofilm.

AB - The crystal violet assay is widely used for biofilm quantitation despite its toxicity and variability. Here, we instead combine fluorescence labelling with the Cytation 5 multi-mode plate reader, to enable simultaneous acquisition of both quantitative and imaging biofilm data. This high-throughput method produces more robust data and provides information about morphology and spatial species organization within the biofilm.

U2 - 10.1016/j.mimet.2021.106343

DO - 10.1016/j.mimet.2021.106343

M3 - Letter

C2 - 34619138

VL - 190

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

M1 - 106343

ER -